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Vectors Uses for Animal Cell Culture

Vectors Uses for Animal Cell Culture
SV 40 vectors, in which early region replacements are carried out, require

pBR 322 vector
SV 40 helper virus
COS cell line
none of these

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Vectors Uses for Animal Cell Culture
Maximum insert size in case of adenovirus vectors is

20 kb
6-8 kb
10-12 kb
4-6 kb

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Vectors Uses for Animal Cell Culture
5-fluoro orotic acid inhibits the growth if one of the following marker is present in the yeast. That is

Trp 1
Lys 2
His 3
Ura 3

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Vectors Uses for Animal Cell Culture
Which of the following can be used for reducing the size of gene inserts containing introns?

Vaccinia virus
none of these
Retro virus
Baculo virus

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Vectors Uses for Animal Cell Culture
Cos cell line is

derivative of non-permissive CV-1 monkey cell line
derivative of permissive CV-1 monkey cell line
cohesive initial site of phage λ
cohesive end site of phage λ

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Vectors Uses for Animal Cell Culture
Using bovine papilloma virus (BPV) derived vectors, permanent cell lines can be obtained carrying a recombinant DNA

integrated only
either episomally or integrated at low copy number
either episomally or integrated at high copy number
episomally only

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