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Vectors Uses for Animal Cell Culture

Vectors Uses for Animal Cell Culture
Which of the following are produced in high copy number?

Both (a) and (b)
YRp
YEp
YCp

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Vectors Uses for Animal Cell Culture
Which one of the following is true about Yeast Episomal plasmids (YEp) vectors?

Allows transformation by using crossing over
Carries autonomously replicating sequence
Has a origin of replication and rep genes
Carries centromere and telomere sequences

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Vectors Uses for Animal Cell Culture
Cos cell line is

derivative of permissive CV-1 monkey cell line
cohesive initial site of phage λ
derivative of non-permissive CV-1 monkey cell line
cohesive end site of phage λ

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Vectors Uses for Animal Cell Culture
Retroviruses consist of

Ds DNA genome
cDNA genome
Ss DNA genome
RNA genome

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Vectors Uses for Animal Cell Culture
Autographa California nuclear polyhedrosis virus that is used as a vector for animal cells is an example of

Retrovirus
Papilloma virus
Baculo virus
Vaccinia virus

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Vectors Uses for Animal Cell Culture
SV 40 vectors, in which early region replacements are carried out, require

none of these
pBR 322 vector
COS cell line
SV 40 helper virus

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