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Vectors Uses for Animal Cell Culture

Vectors Uses for Animal Cell Culture
Which of the following are produced in high copy number?

YCp
Both (a) and (b)
YRp
YEp

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Vectors Uses for Animal Cell Culture
Yeast Replicating plasmids (YRp) carry

centromere
both (a) and (c)
autonomously replicating sequence
telomere

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Vectors Uses for Animal Cell Culture
5-fluoro orotic acid inhibits the growth if one of the following marker is present in the yeast. That is

His 3
Lys 2
Trp 1
Ura 3

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Vectors Uses for Animal Cell Culture
Recombinant SV40 viruses introduce the foreign DNA into cells

with the RNA mediated transfection
without the DNA mediated transfection
with the DNA mediated transfection
without the RNA mediated transfection

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Vectors Uses for Animal Cell Culture
Which one of the following is true about Yeast Episomal plasmids (YEp) vectors?

Carries centromere and telomere sequences
Allows transformation by using crossing over
Has a origin of replication and rep genes
Carries autonomously replicating sequence

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Vectors Uses for Animal Cell Culture
Which one of the following is true about Yeast Centromeric plasmids (YCp) vectors?

All of these
Contains centromeric sequences
Segregates during meiosis and mitosis
Functions as true chromosome

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