Vectors Uses for Animal Cell Culture
Yeast episomal plasmids (YEp) were constructed using

2μm yeast plasmid, fragments of yeast nuclear DNA and E coli vector pMB 9
12μm yeast plasmid and E coli vector pMB 9
2μm yeast plasmid, fragments of yeast nuclear DNA and E coli vector pBR 322
12μm yeast plasmid and E coli vector pBR 322

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Vectors Uses for Animal Cell Culture
Recombinant adeno virus can be created by deleting E1A/E 1B genes that are

transcriptional regulators and are essential for viral replication
important for packaging of viral DNA into viral head
coding for virus coat protein
not necessary for viral replication

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