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Cell Cultures and Characteristics

Cell Cultures and Characteristics
Viable plate count can be obtained using

Spread plate method
Pour plate method
both (a) and (b)
Hemocytometer

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Cell Cultures and Characteristics
In the pour plate method, the mixed culture is diluted directly in tubes of

liquid agar medium
sterile liquid usually water
none of these
both (a) and (b)

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Cell Cultures and Characteristics
The concentration of agar to obtain semi solid media is

0.5% or less
>10%
1.5-20%
>10%but<20%

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Cell Cultures and Characteristics
Which of the following methods is most likely to be quantitative?

Dilution and plating
Wet mount
None of these
Gram staining

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Cell Cultures and Characteristics
Which of the following is used for the proper maintenance and preservation of pure cultures?

Preservation by lyophilization
Periodic transfer to fresh media
Preservation by overlaying cultures with mineral oil
All of these

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Cell Cultures and Characteristics
In the process of freeze drying, a dense cell suspension is placed in small vials and is frozen at

-60 to -78°C
-20 to -30°C
-48 to -58°C
-30 to -48°C

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