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Cell Cultures and Characteristics

Cell Cultures and Characteristics
Selective media facilitate growth of only one kind of organism. Saboraud medium is used to selectively isolate

coliform bacteria
yeasts
gram positive bacteria
acid fast organisms

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Cell Cultures and Characteristics
In the process of freeze drying, a dense cell suspension is placed in small vials and is frozen at

-30 to -48°C
-60 to -78°C
-48 to -58°C
-20 to -30°C

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Cell Cultures and Characteristics
Treponema species from human oral cavity can be selected by using

all of these
pH of the medium
cell size and motility
incubation temperature

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Cell Cultures and Characteristics
Which of the following techniques may be performed quantitatively to determine the number of bacteria of a particular type?

Spread plate
Both (a) and (b)
Pour plate
Streak plate technique

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Cell Cultures and Characteristics
Mac-Conkey medium is an example of

all of these
transport medium
differential medium
enrichment medium

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Cell Cultures and Characteristics
Which of the following is used for the proper maintenance and preservation of pure cultures?

Preservation by overlaying cultures with mineral oil
All of these
Preservation by lyophilization
Periodic transfer to fresh media

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