Cell Cultures and Characteristics
Which of the following is used for the proper maintenance and preservation of pure cultures?

Periodic transfer to fresh media
All of these
Preservation by lyophilization
Preservation by overlaying cultures with mineral oil

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Cell Cultures and Characteristics
Which of the following problem makes it impossible to satisfy all of Koch's postulates?

Microorganism cannot be isolated in pure culture
Microorganism causes serious symptoms in humans
Species of microorganism is in question
Genes from the microorganism cannot be amplified by PCR

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