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Immunological Techniques

Immunological Techniques
Radial Immunodiffusion is similar to

all of these
gel diffusion
Ouchterloney technique
double-diffusion

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Immunological Techniques
What is the ELISA test intended to measure?

Presence of free, circulating virus in the patient
Antibodies directed against HLA molecules
Antigen to HIV only
Antibody to HIV only

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Immunological Techniques
Which of the following is a positively charged matrix for ion exchange chromatography?

CM cellulose
Phosphocellulose
None of these
DEAE cellulose

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Immunological Techniques
DNA- footprinting is a suitable technique for identifying which of the following?

Protein binding site within DNA
Introns within DNA
Particular t-RNA in mixture
Particular mRNA in mixture

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Immunological Techniques
The IPTG is a gratuitous inducers of (β-galactosidase, and x-gal is a chromogenic substrate. When α-complementation occurs which colour is produced?

No colour is produced
Blue
Brown
White

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Immunological Techniques
Immunoaffinity chromatography can be used in biochemical applications to

break down antigen and analyze quantitatively
break down antibody structure
None of these
purify protein antigen

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