Immunological Techniques
Different form of a plasmid, on an agarose gel, after electrophoresis may be in which of the following sequence in decreasing molecular weight?

Open circular, linear plasmid, super coiled plasmid
Linear plasmid, open circular, super coiled plasmid
Open circular, super coiled, linear plasmid DNA
Open circular, super coiled, linear plasmid DNA

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Immunological Techniques
In Rocket Immunodiffusion the length of the rocket is

inversely proportional to the amount of antibody placed in each well
proportional to the amount of antigen placed in each well
proportional to the amount of antibody placed in each well
inversely proportional to the amount of antigen placed in each well

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Immunological Techniques
What would happen if the anti-human Ig-conjugate is not washed free of the well before the substrate is added?

None of these
The ELISA would not develop when the substrate was added
All wells would show uniform over-development due to unbound and excess anti-human Ig enzyme conjugate
The ELISA would develop normally

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