Immunological Techniques
An antigen preparation and an antibody preparation are tested by immunodiffusion. Three bands are found, indicating that

there was more than one antigen
the antibody was impure
there was one antibody and one antigen
the temperature was too high

ANSWER DOWNLOAD EXAMIANS APP

Immunological Techniques
What would happen if the anti-human Ig-conjugate is not washed free of the well before the substrate is added?

The ELISA would not develop when the substrate was added
None of these
The ELISA would develop normally
All wells would show uniform over-development due to unbound and excess anti-human Ig enzyme conjugate

ANSWER DOWNLOAD EXAMIANS APP

Immunological Techniques
Different form of a plasmid, on an agarose gel, after electrophoresis may be in which of the following sequence in decreasing molecular weight?

Linear plasmid, open circular, super coiled plasmid
Open circular, linear plasmid, super coiled plasmid
Open circular, super coiled, linear plasmid DNA
Open circular, super coiled, linear plasmid DNA

ANSWER DOWNLOAD EXAMIANS APP