Immunological Techniques
An antigen preparation and an antibody preparation are tested by immunodiffusion. Three bands are found, indicating that

there was one antibody and one antigen
there was more than one antigen
the antibody was impure
the temperature was too high

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Immunological Techniques
In Rocket Immunodiffusion the length of the rocket is

proportional to the amount of antibody placed in each well
proportional to the amount of antigen placed in each well
inversely proportional to the amount of antibody placed in each well
inversely proportional to the amount of antigen placed in each well

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