Immunological Techniques
An antigen preparation and an antibody preparation are tested by immunodiffusion. Three bands are found, indicating that

the temperature was too high
there was one antibody and one antigen
there was more than one antigen
the antibody was impure

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Immunological Techniques
What would happen if serum is omitted from the ELISA, but all other steps remain same?

Both (a) and (b)
The OD values would be nearly the same as the assay control
Anti-human Ig-conjugate would bind non-specifically to the ELISA plate
Anti-human Ig-conjugate would not bind and be washed away

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