Immunological Techniques
An antigen preparation and an antibody preparation are tested by immunodiffusion. Three bands are found, indicating that

there was more than one antigen
there was one antibody and one antigen
the temperature was too high
the antibody was impure

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Immunological Techniques
In Counter immunoelectrophoresis

electrophoresis will drive the antibody and antigen toward each other
the antibody will migrate towards cathode
electrophoresis will drive the antibody and antigen parallel to each other
the antibody will migrate towards anode

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Immunological Techniques
What would happen if the anti-human Ig-conjugate is not washed free of the well before the substrate is added?

The ELISA would not develop when the substrate was added
All wells would show uniform over-development due to unbound and excess anti-human Ig enzyme conjugate
None of these
The ELISA would develop normally

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