Immunological Techniques
An antigen preparation and an antibody preparation are tested by immunodiffusion. Three bands are found, indicating that

there was one antibody and one antigen
the temperature was too high
there was more than one antigen
the antibody was impure

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Immunological Techniques
Different form of a plasmid, on an agarose gel, after electrophoresis may be in which of the following sequence in decreasing molecular weight?

Open circular, linear plasmid, super coiled plasmid
Linear plasmid, open circular, super coiled plasmid
Open circular, super coiled, linear plasmid DNA
Open circular, super coiled, linear plasmid DNA

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Immunological Techniques
What would happen if the anti-human Ig-conjugate is not washed free of the well before the substrate is added?

The ELISA would not develop when the substrate was added
All wells would show uniform over-development due to unbound and excess anti-human Ig enzyme conjugate
None of these
The ELISA would develop normally

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