Immunological Techniques
An antigen preparation and an antibody preparation are tested by immunodiffusion. Three bands are found, indicating that

there was more than one antigen
the antibody was impure
there was one antibody and one antigen
the temperature was too high

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Immunological Techniques
What would happen if serum is omitted from the ELISA, but all other steps remain same?

Both (a) and (b)
Anti-human Ig-conjugate would not bind and be washed away
The OD values would be nearly the same as the assay control
Anti-human Ig-conjugate would bind non-specifically to the ELISA plate

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