Immunological Techniques
An antigen preparation and an antibody preparation are tested by immunodiffusion. Three bands are found, indicating that

the temperature was too high
there was one antibody and one antigen
the antibody was impure
there was more than one antigen

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Immunological Techniques
What would happen if the anti-human Ig-conjugate is not washed free of the well before the substrate is added?

The ELISA would develop normally
None of these
All wells would show uniform over-development due to unbound and excess anti-human Ig enzyme conjugate
The ELISA would not develop when the substrate was added

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