Immunological Techniques
An antigen preparation and an antibody preparation are tested by immunodiffusion. Three bands are found, indicating that

there was more than one antigen
the temperature was too high
the antibody was impure
there was one antibody and one antigen

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Immunological Techniques
What would happen if the anti-human Ig-conjugate is not washed free of the well before the substrate is added?

The ELISA would develop normally
All wells would show uniform over-development due to unbound and excess anti-human Ig enzyme conjugate
None of these
The ELISA would not develop when the substrate was added

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Immunological Techniques
In Rocket Immunodiffusion the length of the rocket is

proportional to the amount of antibody placed in each well
proportional to the amount of antigen placed in each well
inversely proportional to the amount of antigen placed in each well
inversely proportional to the amount of antibody placed in each well

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