Immunological Techniques
An antigen preparation and an antibody preparation are tested by immunodiffusion. Three bands are found, indicating that

there was one antibody and one antigen
the antibody was impure
the temperature was too high
there was more than one antigen

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Immunological Techniques
What would happen if serum is omitted from the ELISA, but all other steps remain same?

Both (a) and (b)
Anti-human Ig-conjugate would bind non-specifically to the ELISA plate
Anti-human Ig-conjugate would not bind and be washed away
The OD values would be nearly the same as the assay control

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Immunological Techniques
In Counter immunoelectrophoresis

electrophoresis will drive the antibody and antigen toward each other
electrophoresis will drive the antibody and antigen parallel to each other
the antibody will migrate towards cathode
the antibody will migrate towards anode

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