Immunological Techniques
An antigen preparation and an antibody preparation are tested by immunodiffusion. Three bands are found, indicating that

the temperature was too high
there was one antibody and one antigen
there was more than one antigen
the antibody was impure

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Immunological Techniques
In Counter immunoelectrophoresis

the antibody will migrate towards anode
electrophoresis will drive the antibody and antigen parallel to each other
the antibody will migrate towards cathode
electrophoresis will drive the antibody and antigen toward each other

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