Immunological Techniques
Different form of a plasmid, on an agarose gel, after electrophoresis may be in which of the following sequence in decreasing molecular weight?

Open circular, linear plasmid, super coiled plasmid
Open circular, super coiled, linear plasmid DNA
Linear plasmid, open circular, super coiled plasmid
Open circular, super coiled, linear plasmid DNA

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Immunological Techniques
What would happen if the anti-human Ig-conjugate is not washed free of the well before the substrate is added?

The ELISA would not develop when the substrate was added
The ELISA would develop normally
None of these
All wells would show uniform over-development due to unbound and excess anti-human Ig enzyme conjugate

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