Immunological Techniques
Different form of a plasmid, on an agarose gel, after electrophoresis may be in which of the following sequence in decreasing molecular weight?

Linear plasmid, open circular, super coiled plasmid
Open circular, linear plasmid, super coiled plasmid
Open circular, super coiled, linear plasmid DNA
Open circular, super coiled, linear plasmid DNA

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Immunological Techniques
What would happen if serum is omitted from the ELISA, but all other steps remain same?

The OD values would be nearly the same as the assay control
Anti-human Ig-conjugate would not bind and be washed away
Anti-human Ig-conjugate would bind non-specifically to the ELISA plate
Both (a) and (b)

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