Different form of a plasmid, on an agarose gel, after electrophoresis may be in which of the following sequence in decreasing molecular weight?

Immunological Techniques
Different form of a plasmid, on an agarose gel, after electrophoresis may be in which of the following sequence in decreasing molecular weight?

Linear plasmid, open circular, super coiled plasmid

Open circular, super coiled, linear plasmid DNA

Open circular, linear plasmid, super coiled plasmid

Linear plasmid, open circular, super coiled plasmid

Open circular, super coiled, linear plasmid DNA

Open circular, linear plasmid, super coiled plasmid

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Immunological Techniques
What would happen if the anti-human Ig-conjugate is not washed free of the well before the substrate is added?

The ELISA would develop normally

All wells would show uniform over-development due to unbound and excess anti-human Ig enzyme conjugate

None of these

The ELISA would not develop when the substrate was added

The ELISA would develop normally

All wells would show uniform over-development due to unbound and excess anti-human Ig enzyme conjugate

None of these

The ELISA would not develop when the substrate was added

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Immunological Techniques
DNA- footprinting is a suitable technique for identifying which of the following?

Particular t-RNA in mixture

Particular mRNA in mixture

Protein binding site within DNA

Introns within DNA

Particular t-RNA in mixture

Particular mRNA in mixture

Protein binding site within DNA

Introns within DNA

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Immunological Techniques
Agarose gel electrophoresis and pulsed field gel electrophoresis may be used to resolve respectively

20 kb and 2000 kb DNA

10 kb and 1000 kb DNA

2000 kb and 20kb DNA

1000 kb and l0kb DNA

20 kb and 2000 kb DNA

10 kb and 1000 kb DNA

2000 kb and 20kb DNA

1000 kb and l0kb DNA

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Immunological Techniques
An antigen preparation and an antibody preparation are tested by immunodiffusion. Three bands are found, indicating that

there was more than one antigen

the antibody was impure

there was one antibody and one antigen

the temperature was too high

there was more than one antigen

the antibody was impure

there was one antibody and one antigen

the temperature was too high

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Immunological Techniques
What is the ELISA test intended to measure?

Presence of free, circulating virus in the patient

Antibodies directed against HLA molecules

Antigen to HIV only

Antibody to HIV only

Presence of free, circulating virus in the patient

Antibodies directed against HLA molecules

Antigen to HIV only

Antibody to HIV only

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Immunological Techniques

Immunological Techniques Different form of a plasmid, on an agarose gel, after electrophoresis may be in which of the following sequence in decreasing molecular weight?

Immunological Techniques What would happen if the anti-human Ig-conjugate is not washed free of the well before the substrate is added?

Immunological Techniques DNA- footprinting is a suitable technique for identifying which of the following?

Immunological Techniques Agarose gel electrophoresis and pulsed field gel electrophoresis may be used to resolve respectively

Immunological Techniques An antigen preparation and an antibody preparation are tested by immunodiffusion. Three bands are found, indicating that

Immunological Techniques What is the ELISA test intended to measure?

Immunological Techniques
Different form of a plasmid, on an agarose gel, after electrophoresis may be in which of the following sequence in decreasing molecular weight?

Immunological Techniques
What would happen if the anti-human Ig-conjugate is not washed free of the well before the substrate is added?

Immunological Techniques
DNA- footprinting is a suitable technique for identifying which of the following?

Immunological Techniques
Agarose gel electrophoresis and pulsed field gel electrophoresis may be used to resolve respectively

Immunological Techniques
An antigen preparation and an antibody preparation are tested by immunodiffusion. Three bands are found, indicating that

Immunological Techniques
What is the ELISA test intended to measure?