Immunological Techniques
The competitive immunoassay can be used

commonly to detect trace amounts of drugs.
to detect very small amounts of antigen
to detect antibody associated with allergies (IgE)
both (a) and (b)

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Immunological Techniques
What would happen if the anti-human Ig-conjugate is not washed free of the well before the substrate is added?

All wells would show uniform over-development due to unbound and excess anti-human Ig enzyme conjugate
None of these
The ELISA would not develop when the substrate was added
The ELISA would develop normally

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