Immunological Techniques
The competitive immunoassay can be used

to detect very small amounts of antigen
commonly to detect trace amounts of drugs.
both (a) and (b)
to detect antibody associated with allergies (IgE)

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Immunological Techniques
What would happen if serum is omitted from the ELISA, but all other steps remain same?

Both (a) and (b)
The OD values would be nearly the same as the assay control
Anti-human Ig-conjugate would bind non-specifically to the ELISA plate
Anti-human Ig-conjugate would not bind and be washed away

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