Immunological Techniques
The competitive immunoassay can be used

both (a) and (b)
to detect antibody associated with allergies (IgE)
to detect very small amounts of antigen
commonly to detect trace amounts of drugs.

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Immunological Techniques
What would happen if the anti-human Ig-conjugate is not washed free of the well before the substrate is added?

None of these
All wells would show uniform over-development due to unbound and excess anti-human Ig enzyme conjugate
The ELISA would develop normally
The ELISA would not develop when the substrate was added

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