Immunological Techniques RAST test (radio allergosorbent test) is often used to detect antibodies associated with allergies (IgE) bacteriophages antigen associated with allergies (IgE) None of these antibodies associated with allergies (IgE) bacteriophages antigen associated with allergies (IgE) None of these ANSWER DOWNLOAD EXAMIANS APP
Immunological Techniques What would happen if serum is omitted from the ELISA, but all other steps remain same? Anti-human Ig-conjugate would not bind and be washed away Both (a) and (b) The OD values would be nearly the same as the assay control Anti-human Ig-conjugate would bind non-specifically to the ELISA plate Anti-human Ig-conjugate would not bind and be washed away Both (a) and (b) The OD values would be nearly the same as the assay control Anti-human Ig-conjugate would bind non-specifically to the ELISA plate ANSWER DOWNLOAD EXAMIANS APP
Immunological Techniques What would happen if the anti-human Ig-conjugate is not washed free of the well before the substrate is added? All wells would show uniform over-development due to unbound and excess anti-human Ig enzyme conjugate None of these The ELISA would develop normally The ELISA would not develop when the substrate was added All wells would show uniform over-development due to unbound and excess anti-human Ig enzyme conjugate None of these The ELISA would develop normally The ELISA would not develop when the substrate was added ANSWER DOWNLOAD EXAMIANS APP
Immunological Techniques What radioactive molecule is used to end-label an oligonucleotide? Any of these (alpha)-32P-ATP (gamma)-32P-ATP (alpha)-32P-dUTP Any of these (alpha)-32P-ATP (gamma)-32P-ATP (alpha)-32P-dUTP ANSWER DOWNLOAD EXAMIANS APP
Immunological Techniques An antigen preparation and an antibody preparation are tested by immunodiffusion. Three bands are found, indicating that there was more than one antigen the temperature was too high there was one antibody and one antigen the antibody was impure there was more than one antigen the temperature was too high there was one antibody and one antigen the antibody was impure ANSWER DOWNLOAD EXAMIANS APP
Immunological Techniques In Rocket Immunodiffusion the length of the rocket is inversely proportional to the amount of antigen placed in each well proportional to the amount of antigen placed in each well proportional to the amount of antibody placed in each well inversely proportional to the amount of antibody placed in each well inversely proportional to the amount of antigen placed in each well proportional to the amount of antigen placed in each well proportional to the amount of antibody placed in each well inversely proportional to the amount of antibody placed in each well ANSWER DOWNLOAD EXAMIANS APP
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