Immunological Techniques
The IPTG is a gratuitous inducers of (β-galactosidase, and x-gal is a chromogenic substrate. When α-complementation occurs which colour is produced?

White
No colour is produced
Blue
Brown

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Immunological Techniques
What would happen if serum is omitted from the ELISA, but all other steps remain same?

Anti-human Ig-conjugate would bind non-specifically to the ELISA plate
Anti-human Ig-conjugate would not bind and be washed away
The OD values would be nearly the same as the assay control
Both (a) and (b)

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