Immunological Techniques The IPTG is a gratuitous inducers of (β-galactosidase, and x-gal is a chromogenic substrate. When α-complementation occurs which colour is produced? Brown Blue White No colour is produced Brown Blue White No colour is produced ANSWER DOWNLOAD EXAMIANS APP
Immunological Techniques What would happen if serum is omitted from the ELISA, but all other steps remain same? Anti-human Ig-conjugate would bind non-specifically to the ELISA plate The OD values would be nearly the same as the assay control Anti-human Ig-conjugate would not bind and be washed away Both (a) and (b) Anti-human Ig-conjugate would bind non-specifically to the ELISA plate The OD values would be nearly the same as the assay control Anti-human Ig-conjugate would not bind and be washed away Both (a) and (b) ANSWER DOWNLOAD EXAMIANS APP
Immunological Techniques RAST test (radio allergosorbent test) is often used to detect antigen associated with allergies (IgE) bacteriophages antibodies associated with allergies (IgE) None of these antigen associated with allergies (IgE) bacteriophages antibodies associated with allergies (IgE) None of these ANSWER DOWNLOAD EXAMIANS APP
Immunological Techniques What is the ELISA test intended to measure? Antibody to HIV only Antibodies directed against HLA molecules Antigen to HIV only Presence of free, circulating virus in the patient Antibody to HIV only Antibodies directed against HLA molecules Antigen to HIV only Presence of free, circulating virus in the patient ANSWER DOWNLOAD EXAMIANS APP
Immunological Techniques An antigen preparation and an antibody preparation are tested by immunodiffusion. Three bands are found, indicating that the antibody was impure the temperature was too high there was one antibody and one antigen there was more than one antigen the antibody was impure the temperature was too high there was one antibody and one antigen there was more than one antigen ANSWER DOWNLOAD EXAMIANS APP
Immunological Techniques Different form of a plasmid, on an agarose gel, after electrophoresis may be in which of the following sequence in decreasing molecular weight? Open circular, linear plasmid, super coiled plasmid Open circular, super coiled, linear plasmid DNA Linear plasmid, open circular, super coiled plasmid Open circular, super coiled, linear plasmid DNA Open circular, linear plasmid, super coiled plasmid Open circular, super coiled, linear plasmid DNA Linear plasmid, open circular, super coiled plasmid Open circular, super coiled, linear plasmid DNA ANSWER DOWNLOAD EXAMIANS APP