Immunological Techniques The IPTG is a gratuitous inducers of (β-galactosidase, and x-gal is a chromogenic substrate. When α-complementation occurs which colour is produced? Blue Brown No colour is produced White Blue Brown No colour is produced White ANSWER DOWNLOAD EXAMIANS APP
Immunological Techniques What would happen if the anti-human Ig-conjugate is not washed free of the well before the substrate is added? None of these The ELISA would develop normally The ELISA would not develop when the substrate was added All wells would show uniform over-development due to unbound and excess anti-human Ig enzyme conjugate None of these The ELISA would develop normally The ELISA would not develop when the substrate was added All wells would show uniform over-development due to unbound and excess anti-human Ig enzyme conjugate ANSWER DOWNLOAD EXAMIANS APP
Immunological Techniques Different form of a plasmid, on an agarose gel, after electrophoresis may be in which of the following sequence in decreasing molecular weight? Open circular, super coiled, linear plasmid DNA Open circular, super coiled, linear plasmid DNA Linear plasmid, open circular, super coiled plasmid Open circular, linear plasmid, super coiled plasmid Open circular, super coiled, linear plasmid DNA Open circular, super coiled, linear plasmid DNA Linear plasmid, open circular, super coiled plasmid Open circular, linear plasmid, super coiled plasmid ANSWER DOWNLOAD EXAMIANS APP
Immunological Techniques What is approximatively the half life of 32-Phosphorus? 28 days 14 days 30 days 42 days 28 days 14 days 30 days 42 days ANSWER DOWNLOAD EXAMIANS APP
Immunological Techniques What is the ELISA test intended to measure? Antibody to HIV only Antibodies directed against HLA molecules Antigen to HIV only Presence of free, circulating virus in the patient Antibody to HIV only Antibodies directed against HLA molecules Antigen to HIV only Presence of free, circulating virus in the patient ANSWER DOWNLOAD EXAMIANS APP
Immunological Techniques What would happen if serum is omitted from the ELISA, but all other steps remain same? The OD values would be nearly the same as the assay control Both (a) and (b) Anti-human Ig-conjugate would not bind and be washed away Anti-human Ig-conjugate would bind non-specifically to the ELISA plate The OD values would be nearly the same as the assay control Both (a) and (b) Anti-human Ig-conjugate would not bind and be washed away Anti-human Ig-conjugate would bind non-specifically to the ELISA plate ANSWER DOWNLOAD EXAMIANS APP
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