Immunological Techniques The IPTG is a gratuitous inducers of (β-galactosidase, and x-gal is a chromogenic substrate. When α-complementation occurs which colour is produced? White No colour is produced Brown Blue White No colour is produced Brown Blue ANSWER DOWNLOAD EXAMIANS APP
Immunological Techniques Different form of a plasmid, on an agarose gel, after electrophoresis may be in which of the following sequence in decreasing molecular weight? Linear plasmid, open circular, super coiled plasmid Open circular, super coiled, linear plasmid DNA Open circular, linear plasmid, super coiled plasmid Open circular, super coiled, linear plasmid DNA Linear plasmid, open circular, super coiled plasmid Open circular, super coiled, linear plasmid DNA Open circular, linear plasmid, super coiled plasmid Open circular, super coiled, linear plasmid DNA ANSWER DOWNLOAD EXAMIANS APP
Immunological Techniques What would happen if serum is omitted from the ELISA, but all other steps remain same? The OD values would be nearly the same as the assay control Anti-human Ig-conjugate would not bind and be washed away Both (a) and (b) Anti-human Ig-conjugate would bind non-specifically to the ELISA plate The OD values would be nearly the same as the assay control Anti-human Ig-conjugate would not bind and be washed away Both (a) and (b) Anti-human Ig-conjugate would bind non-specifically to the ELISA plate ANSWER DOWNLOAD EXAMIANS APP
Immunological Techniques Agarose gel electrophoresis and pulsed field gel electrophoresis may be used to resolve respectively 10 kb and 1000 kb DNA 2000 kb and 20kb DNA 20 kb and 2000 kb DNA 1000 kb and l0kb DNA 10 kb and 1000 kb DNA 2000 kb and 20kb DNA 20 kb and 2000 kb DNA 1000 kb and l0kb DNA ANSWER DOWNLOAD EXAMIANS APP
Immunological Techniques DNA- footprinting is a suitable technique for identifying which of the following? Introns within DNA Particular t-RNA in mixture Particular mRNA in mixture Protein binding site within DNA Introns within DNA Particular t-RNA in mixture Particular mRNA in mixture Protein binding site within DNA ANSWER DOWNLOAD EXAMIANS APP
Immunological Techniques What radioactive molecule is used to end-label an oligonucleotide? (alpha)-32P-ATP (alpha)-32P-dUTP (gamma)-32P-ATP Any of these (alpha)-32P-ATP (alpha)-32P-dUTP (gamma)-32P-ATP Any of these ANSWER DOWNLOAD EXAMIANS APP
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