Immunological Techniques
The IPTG is a gratuitous inducers of (β-galactosidase, and x-gal is a chromogenic substrate. When α-complementation occurs which colour is produced?

No colour is produced
Brown
Blue
White

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Immunological Techniques
What would happen if the anti-human Ig-conjugate is not washed free of the well before the substrate is added?

The ELISA would not develop when the substrate was added
All wells would show uniform over-development due to unbound and excess anti-human Ig enzyme conjugate
The ELISA would develop normally
None of these

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