Immunological Techniques The IPTG is a gratuitous inducers of (β-galactosidase, and x-gal is a chromogenic substrate. When α-complementation occurs which colour is produced? Brown No colour is produced Blue White Brown No colour is produced Blue White ANSWER DOWNLOAD EXAMIANS APP
Immunological Techniques Which of the following radioactive element is generally involved in RIA? Iodine-125 Carbon-14 Tritium All of these Iodine-125 Carbon-14 Tritium All of these ANSWER DOWNLOAD EXAMIANS APP
Immunological Techniques What is the ELISA test intended to measure? Antibodies directed against HLA molecules Presence of free, circulating virus in the patient Antibody to HIV only Antigen to HIV only Antibodies directed against HLA molecules Presence of free, circulating virus in the patient Antibody to HIV only Antigen to HIV only ANSWER DOWNLOAD EXAMIANS APP
Immunological Techniques What would happen if serum is omitted from the ELISA, but all other steps remain same? The OD values would be nearly the same as the assay control Anti-human Ig-conjugate would not bind and be washed away Anti-human Ig-conjugate would bind non-specifically to the ELISA plate Both (a) and (b) The OD values would be nearly the same as the assay control Anti-human Ig-conjugate would not bind and be washed away Anti-human Ig-conjugate would bind non-specifically to the ELISA plate Both (a) and (b) ANSWER DOWNLOAD EXAMIANS APP
Immunological Techniques Agarose gel electrophoresis and pulsed field gel electrophoresis may be used to resolve respectively 1000 kb and l0kb DNA 20 kb and 2000 kb DNA 2000 kb and 20kb DNA 10 kb and 1000 kb DNA 1000 kb and l0kb DNA 20 kb and 2000 kb DNA 2000 kb and 20kb DNA 10 kb and 1000 kb DNA ANSWER DOWNLOAD EXAMIANS APP
Immunological Techniques What radioactive molecule is used to end-label an oligonucleotide? (gamma)-32P-ATP (alpha)-32P-ATP Any of these (alpha)-32P-dUTP (gamma)-32P-ATP (alpha)-32P-ATP Any of these (alpha)-32P-dUTP ANSWER DOWNLOAD EXAMIANS APP
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