Immunological Techniques The IPTG is a gratuitous inducers of (β-galactosidase, and x-gal is a chromogenic substrate. When α-complementation occurs which colour is produced? White No colour is produced Blue Brown White No colour is produced Blue Brown ANSWER DOWNLOAD EXAMIANS APP
Immunological Techniques What would happen if serum is omitted from the ELISA, but all other steps remain same? Anti-human Ig-conjugate would bind non-specifically to the ELISA plate Anti-human Ig-conjugate would not bind and be washed away The OD values would be nearly the same as the assay control Both (a) and (b) Anti-human Ig-conjugate would bind non-specifically to the ELISA plate Anti-human Ig-conjugate would not bind and be washed away The OD values would be nearly the same as the assay control Both (a) and (b) ANSWER DOWNLOAD EXAMIANS APP
Immunological Techniques Which of the following is a positively charged matrix for ion exchange chromatography? None of these DEAE cellulose CM cellulose Phosphocellulose None of these DEAE cellulose CM cellulose Phosphocellulose ANSWER DOWNLOAD EXAMIANS APP
Immunological Techniques Immunoaffinity chromatography can be used in biochemical applications to break down antigen and analyze quantitatively purify protein antigen None of these break down antibody structure break down antigen and analyze quantitatively purify protein antigen None of these break down antibody structure ANSWER DOWNLOAD EXAMIANS APP
Immunological Techniques Agarose a porous matrix used in various molecular biology technique is made by repetitive units of agarobiose none of these maltose agar agarobiose none of these maltose agar ANSWER DOWNLOAD EXAMIANS APP
Immunological Techniques An antigen preparation and an antibody preparation are tested by immunodiffusion. Three bands are found, indicating that there was one antibody and one antigen the antibody was impure the temperature was too high there was more than one antigen there was one antibody and one antigen the antibody was impure the temperature was too high there was more than one antigen ANSWER DOWNLOAD EXAMIANS APP