Immunological Techniques
Agarose gel electrophoresis and pulsed field gel electrophoresis may be used to resolve respectively

2000 kb and 20kb DNA
1000 kb and l0kb DNA
20 kb and 2000 kb DNA
10 kb and 1000 kb DNA

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Immunological Techniques
What would happen if serum is omitted from the ELISA, but all other steps remain same?

The OD values would be nearly the same as the assay control
Both (a) and (b)
Anti-human Ig-conjugate would not bind and be washed away
Anti-human Ig-conjugate would bind non-specifically to the ELISA plate

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Immunological Techniques
What would happen if the anti-human Ig-conjugate is not washed free of the well before the substrate is added?

All wells would show uniform over-development due to unbound and excess anti-human Ig enzyme conjugate
The ELISA would develop normally
The ELISA would not develop when the substrate was added
None of these

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