Immunological Techniques
Agarose gel electrophoresis and pulsed field gel electrophoresis may be used to resolve respectively

2000 kb and 20kb DNA
20 kb and 2000 kb DNA
1000 kb and l0kb DNA
10 kb and 1000 kb DNA

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Immunological Techniques
What would happen if serum is omitted from the ELISA, but all other steps remain same?

Anti-human Ig-conjugate would not bind and be washed away
Both (a) and (b)
The OD values would be nearly the same as the assay control
Anti-human Ig-conjugate would bind non-specifically to the ELISA plate

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Immunological Techniques
Different form of a plasmid, on an agarose gel, after electrophoresis may be in which of the following sequence in decreasing molecular weight?

Open circular, linear plasmid, super coiled plasmid
Open circular, super coiled, linear plasmid DNA
Open circular, super coiled, linear plasmid DNA
Linear plasmid, open circular, super coiled plasmid

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