Immunological Techniques
Agarose gel electrophoresis and pulsed field gel electrophoresis may be used to resolve respectively

20 kb and 2000 kb DNA
2000 kb and 20kb DNA
1000 kb and l0kb DNA
10 kb and 1000 kb DNA

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Immunological Techniques
Different form of a plasmid, on an agarose gel, after electrophoresis may be in which of the following sequence in decreasing molecular weight?

Linear plasmid, open circular, super coiled plasmid
Open circular, super coiled, linear plasmid DNA
Open circular, linear plasmid, super coiled plasmid
Open circular, super coiled, linear plasmid DNA

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Immunological Techniques
What would happen if serum is omitted from the ELISA, but all other steps remain same?

Anti-human Ig-conjugate would not bind and be washed away
The OD values would be nearly the same as the assay control
Both (a) and (b)
Anti-human Ig-conjugate would bind non-specifically to the ELISA plate

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Immunological Techniques
In Rocket Immunodiffusion the length of the rocket is

inversely proportional to the amount of antigen placed in each well
inversely proportional to the amount of antibody placed in each well
proportional to the amount of antibody placed in each well
proportional to the amount of antigen placed in each well

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