Immunological Techniques
Immunoaffinity chromatography can be used in biochemical applications to

None of these
break down antigen and analyze quantitatively
purify protein antigen
break down antibody structure

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Immunological Techniques
What would happen if the anti-human Ig-conjugate is not washed free of the well before the substrate is added?

All wells would show uniform over-development due to unbound and excess anti-human Ig enzyme conjugate
The ELISA would develop normally
None of these
The ELISA would not develop when the substrate was added

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Immunological Techniques
What would happen if serum is omitted from the ELISA, but all other steps remain same?

The OD values would be nearly the same as the assay control
Anti-human Ig-conjugate would bind non-specifically to the ELISA plate
Anti-human Ig-conjugate would not bind and be washed away
Both (a) and (b)

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