Immunological Techniques Immunoaffinity chromatography can be used in biochemical applications to None of these break down antibody structure break down antigen and analyze quantitatively purify protein antigen None of these break down antibody structure break down antigen and analyze quantitatively purify protein antigen ANSWER DOWNLOAD EXAMIANS APP
Immunological Techniques What would happen if the anti-human Ig-conjugate is not washed free of the well before the substrate is added? All wells would show uniform over-development due to unbound and excess anti-human Ig enzyme conjugate None of these The ELISA would develop normally The ELISA would not develop when the substrate was added All wells would show uniform over-development due to unbound and excess anti-human Ig enzyme conjugate None of these The ELISA would develop normally The ELISA would not develop when the substrate was added ANSWER DOWNLOAD EXAMIANS APP
Immunological Techniques DNA- footprinting is a suitable technique for identifying which of the following? Particular mRNA in mixture Protein binding site within DNA Particular t-RNA in mixture Introns within DNA Particular mRNA in mixture Protein binding site within DNA Particular t-RNA in mixture Introns within DNA ANSWER DOWNLOAD EXAMIANS APP
Immunological Techniques Agarose gel electrophoresis and pulsed field gel electrophoresis may be used to resolve respectively 1000 kb and l0kb DNA 10 kb and 1000 kb DNA 2000 kb and 20kb DNA 20 kb and 2000 kb DNA 1000 kb and l0kb DNA 10 kb and 1000 kb DNA 2000 kb and 20kb DNA 20 kb and 2000 kb DNA ANSWER DOWNLOAD EXAMIANS APP
Immunological Techniques The IPTG is a gratuitous inducers of (β-galactosidase, and x-gal is a chromogenic substrate. When α-complementation occurs which colour is produced? Blue No colour is produced White Brown Blue No colour is produced White Brown ANSWER DOWNLOAD EXAMIANS APP
Immunological Techniques What would happen if serum is omitted from the ELISA, but all other steps remain same? The OD values would be nearly the same as the assay control Anti-human Ig-conjugate would bind non-specifically to the ELISA plate Anti-human Ig-conjugate would not bind and be washed away Both (a) and (b) The OD values would be nearly the same as the assay control Anti-human Ig-conjugate would bind non-specifically to the ELISA plate Anti-human Ig-conjugate would not bind and be washed away Both (a) and (b) ANSWER DOWNLOAD EXAMIANS APP
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