Immunological Techniques DNA- footprinting is a suitable technique for identifying which of the following? Protein binding site within DNA Particular t-RNA in mixture Particular mRNA in mixture Introns within DNA Protein binding site within DNA Particular t-RNA in mixture Particular mRNA in mixture Introns within DNA ANSWER DOWNLOAD EXAMIANS APP
Immunological Techniques What would happen if the anti-human Ig-conjugate is not washed free of the well before the substrate is added? None of these The ELISA would not develop when the substrate was added All wells would show uniform over-development due to unbound and excess anti-human Ig enzyme conjugate The ELISA would develop normally None of these The ELISA would not develop when the substrate was added All wells would show uniform over-development due to unbound and excess anti-human Ig enzyme conjugate The ELISA would develop normally ANSWER DOWNLOAD EXAMIANS APP
Immunological Techniques Different form of a plasmid, on an agarose gel, after electrophoresis may be in which of the following sequence in decreasing molecular weight? Linear plasmid, open circular, super coiled plasmid Open circular, super coiled, linear plasmid DNA Open circular, super coiled, linear plasmid DNA Open circular, linear plasmid, super coiled plasmid Linear plasmid, open circular, super coiled plasmid Open circular, super coiled, linear plasmid DNA Open circular, super coiled, linear plasmid DNA Open circular, linear plasmid, super coiled plasmid ANSWER DOWNLOAD EXAMIANS APP
Immunological Techniques An antigen preparation and an antibody preparation are tested by immunodiffusion. Three bands are found, indicating that the antibody was impure there was one antibody and one antigen there was more than one antigen the temperature was too high the antibody was impure there was one antibody and one antigen there was more than one antigen the temperature was too high ANSWER DOWNLOAD EXAMIANS APP
Immunological Techniques The IPTG is a gratuitous inducers of (β-galactosidase, and x-gal is a chromogenic substrate. When α-complementation occurs which colour is produced? No colour is produced White Brown Blue No colour is produced White Brown Blue ANSWER DOWNLOAD EXAMIANS APP
Immunological Techniques Immunoelectrophoresis techniques are designed to separate the mixture components from each other by using electrophoresis after reaction with antibody after reaction with antigen prior to reaction with antigen prior to reaction with antibody after reaction with antibody after reaction with antigen prior to reaction with antigen prior to reaction with antibody ANSWER DOWNLOAD EXAMIANS APP