Immunological Techniques Which of the following is a positively charged matrix for ion exchange chromatography? CM cellulose Phosphocellulose DEAE cellulose None of these CM cellulose Phosphocellulose DEAE cellulose None of these ANSWER DOWNLOAD EXAMIANS APP
Immunological Techniques DNA- footprinting is a suitable technique for identifying which of the following? Particular mRNA in mixture Protein binding site within DNA Particular t-RNA in mixture Introns within DNA Particular mRNA in mixture Protein binding site within DNA Particular t-RNA in mixture Introns within DNA ANSWER DOWNLOAD EXAMIANS APP
Immunological Techniques What would happen if serum is omitted from the ELISA, but all other steps remain same? Both (a) and (b) The OD values would be nearly the same as the assay control Anti-human Ig-conjugate would bind non-specifically to the ELISA plate Anti-human Ig-conjugate would not bind and be washed away Both (a) and (b) The OD values would be nearly the same as the assay control Anti-human Ig-conjugate would bind non-specifically to the ELISA plate Anti-human Ig-conjugate would not bind and be washed away ANSWER DOWNLOAD EXAMIANS APP
Immunological Techniques What would happen if the anti-human Ig-conjugate is not washed free of the well before the substrate is added? None of these The ELISA would not develop when the substrate was added The ELISA would develop normally All wells would show uniform over-development due to unbound and excess anti-human Ig enzyme conjugate None of these The ELISA would not develop when the substrate was added The ELISA would develop normally All wells would show uniform over-development due to unbound and excess anti-human Ig enzyme conjugate ANSWER DOWNLOAD EXAMIANS APP
Immunological Techniques What radioactive molecule is used to end-label an oligonucleotide? (alpha)-32P-ATP (alpha)-32P-dUTP (gamma)-32P-ATP Any of these (alpha)-32P-ATP (alpha)-32P-dUTP (gamma)-32P-ATP Any of these ANSWER DOWNLOAD EXAMIANS APP
Immunological Techniques Immunoelectrophoresis techniques are designed to separate the mixture components from each other by using electrophoresis after reaction with antigen after reaction with antibody prior to reaction with antibody prior to reaction with antigen after reaction with antigen after reaction with antibody prior to reaction with antibody prior to reaction with antigen ANSWER DOWNLOAD EXAMIANS APP
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