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Immunological Techniques

Immunological Techniques
Which of the following is a positively charged matrix for ion exchange chromatography?

Phosphocellulose
None of these
CM cellulose
DEAE cellulose

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Immunological Techniques
DNA- footprinting is a suitable technique for identifying which of the following?

Protein binding site within DNA
Particular t-RNA in mixture
Particular mRNA in mixture
Introns within DNA

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Immunological Techniques
The competitive immunoassay can be used

commonly to detect trace amounts of drugs.
to detect antibody associated with allergies (IgE)
to detect very small amounts of antigen
both (a) and (b)

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Immunological Techniques
Immunoaffinity chromatography can be used in biochemical applications to

break down antigen and analyze quantitatively
None of these
break down antibody structure
purify protein antigen

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Immunological Techniques
What is the ELISA test intended to measure?

Presence of free, circulating virus in the patient
Antibody to HIV only
Antigen to HIV only
Antibodies directed against HLA molecules

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Immunological Techniques
What radioactive molecule is used to end-label an oligonucleotide?

(alpha)-32P-dUTP
(gamma)-32P-ATP
Any of these
(alpha)-32P-ATP

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