Immunological Techniques What is the ELISA test intended to measure? Antigen to HIV only Presence of free, circulating virus in the patient Antibodies directed against HLA molecules Antibody to HIV only Antigen to HIV only Presence of free, circulating virus in the patient Antibodies directed against HLA molecules Antibody to HIV only ANSWER DOWNLOAD EXAMIANS APP
Immunological Techniques Immunoaffinity chromatography can be used in biochemical applications to None of these purify protein antigen break down antigen and analyze quantitatively break down antibody structure None of these purify protein antigen break down antigen and analyze quantitatively break down antibody structure ANSWER DOWNLOAD EXAMIANS APP
Immunological Techniques What would happen if the anti-human Ig-conjugate is not washed free of the well before the substrate is added? The ELISA would not develop when the substrate was added All wells would show uniform over-development due to unbound and excess anti-human Ig enzyme conjugate None of these The ELISA would develop normally The ELISA would not develop when the substrate was added All wells would show uniform over-development due to unbound and excess anti-human Ig enzyme conjugate None of these The ELISA would develop normally ANSWER DOWNLOAD EXAMIANS APP
Immunological Techniques Agarose a porous matrix used in various molecular biology technique is made by repetitive units of maltose agarobiose none of these agar maltose agarobiose none of these agar ANSWER DOWNLOAD EXAMIANS APP
Immunological Techniques RAST test (radio allergosorbent test) is often used to detect antibodies associated with allergies (IgE) antigen associated with allergies (IgE) bacteriophages None of these antibodies associated with allergies (IgE) antigen associated with allergies (IgE) bacteriophages None of these ANSWER DOWNLOAD EXAMIANS APP
Immunological Techniques What would happen if serum is omitted from the ELISA, but all other steps remain same? The OD values would be nearly the same as the assay control Both (a) and (b) Anti-human Ig-conjugate would bind non-specifically to the ELISA plate Anti-human Ig-conjugate would not bind and be washed away The OD values would be nearly the same as the assay control Both (a) and (b) Anti-human Ig-conjugate would bind non-specifically to the ELISA plate Anti-human Ig-conjugate would not bind and be washed away ANSWER DOWNLOAD EXAMIANS APP