Immunological Techniques What is the ELISA test intended to measure? Antibodies directed against HLA molecules Presence of free, circulating virus in the patient Antigen to HIV only Antibody to HIV only Antibodies directed against HLA molecules Presence of free, circulating virus in the patient Antigen to HIV only Antibody to HIV only ANSWER DOWNLOAD EXAMIANS APP
Immunological Techniques What would happen if the anti-human Ig-conjugate is not washed free of the well before the substrate is added? The ELISA would not develop when the substrate was added All wells would show uniform over-development due to unbound and excess anti-human Ig enzyme conjugate None of these The ELISA would develop normally The ELISA would not develop when the substrate was added All wells would show uniform over-development due to unbound and excess anti-human Ig enzyme conjugate None of these The ELISA would develop normally ANSWER DOWNLOAD EXAMIANS APP
Immunological Techniques What would happen if serum is omitted from the ELISA, but all other steps remain same? Anti-human Ig-conjugate would bind non-specifically to the ELISA plate Both (a) and (b) Anti-human Ig-conjugate would not bind and be washed away The OD values would be nearly the same as the assay control Anti-human Ig-conjugate would bind non-specifically to the ELISA plate Both (a) and (b) Anti-human Ig-conjugate would not bind and be washed away The OD values would be nearly the same as the assay control ANSWER DOWNLOAD EXAMIANS APP
Immunological Techniques The IPTG is a gratuitous inducers of (β-galactosidase, and x-gal is a chromogenic substrate. When α-complementation occurs which colour is produced? Blue No colour is produced White Brown Blue No colour is produced White Brown ANSWER DOWNLOAD EXAMIANS APP
Immunological Techniques What radioactive molecule is used to end-label an oligonucleotide? (gamma)-32P-ATP Any of these (alpha)-32P-ATP (alpha)-32P-dUTP (gamma)-32P-ATP Any of these (alpha)-32P-ATP (alpha)-32P-dUTP ANSWER DOWNLOAD EXAMIANS APP
Immunological Techniques Immunoelectrophoresis techniques are designed to separate the mixture components from each other by using electrophoresis after reaction with antigen after reaction with antibody prior to reaction with antibody prior to reaction with antigen after reaction with antigen after reaction with antibody prior to reaction with antibody prior to reaction with antigen ANSWER DOWNLOAD EXAMIANS APP
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