Immunological Techniques Which of the following radioactive element is generally involved in RIA? Tritium Iodine-125 Carbon-14 All of these Tritium Iodine-125 Carbon-14 All of these ANSWER DOWNLOAD EXAMIANS APP
Immunological Techniques In Rocket Immunodiffusion the length of the rocket is inversely proportional to the amount of antibody placed in each well inversely proportional to the amount of antigen placed in each well proportional to the amount of antibody placed in each well proportional to the amount of antigen placed in each well inversely proportional to the amount of antibody placed in each well inversely proportional to the amount of antigen placed in each well proportional to the amount of antibody placed in each well proportional to the amount of antigen placed in each well ANSWER DOWNLOAD EXAMIANS APP
Immunological Techniques The IPTG is a gratuitous inducers of (β-galactosidase, and x-gal is a chromogenic substrate. When α-complementation occurs which colour is produced? Brown Blue White No colour is produced Brown Blue White No colour is produced ANSWER DOWNLOAD EXAMIANS APP
Immunological Techniques What would happen if serum is omitted from the ELISA, but all other steps remain same? Anti-human Ig-conjugate would bind non-specifically to the ELISA plate The OD values would be nearly the same as the assay control Both (a) and (b) Anti-human Ig-conjugate would not bind and be washed away Anti-human Ig-conjugate would bind non-specifically to the ELISA plate The OD values would be nearly the same as the assay control Both (a) and (b) Anti-human Ig-conjugate would not bind and be washed away ANSWER DOWNLOAD EXAMIANS APP
Immunological Techniques What would happen if the anti-human Ig-conjugate is not washed free of the well before the substrate is added? None of these All wells would show uniform over-development due to unbound and excess anti-human Ig enzyme conjugate The ELISA would not develop when the substrate was added The ELISA would develop normally None of these All wells would show uniform over-development due to unbound and excess anti-human Ig enzyme conjugate The ELISA would not develop when the substrate was added The ELISA would develop normally ANSWER DOWNLOAD EXAMIANS APP
Immunological Techniques What is the ELISA test intended to measure? Antibodies directed against HLA molecules Antigen to HIV only Antibody to HIV only Presence of free, circulating virus in the patient Antibodies directed against HLA molecules Antigen to HIV only Antibody to HIV only Presence of free, circulating virus in the patient ANSWER DOWNLOAD EXAMIANS APP
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