Immunological Techniques Which of the following radioactive element is generally involved in RIA? Tritium Iodine-125 All of these Carbon-14 Tritium Iodine-125 All of these Carbon-14 ANSWER DOWNLOAD EXAMIANS APP
Immunological Techniques What would happen if the anti-human Ig-conjugate is not washed free of the well before the substrate is added? The ELISA would not develop when the substrate was added None of these All wells would show uniform over-development due to unbound and excess anti-human Ig enzyme conjugate The ELISA would develop normally The ELISA would not develop when the substrate was added None of these All wells would show uniform over-development due to unbound and excess anti-human Ig enzyme conjugate The ELISA would develop normally ANSWER DOWNLOAD EXAMIANS APP
Immunological Techniques What would happen if serum is omitted from the ELISA, but all other steps remain same? Anti-human Ig-conjugate would not bind and be washed away The OD values would be nearly the same as the assay control Both (a) and (b) Anti-human Ig-conjugate would bind non-specifically to the ELISA plate Anti-human Ig-conjugate would not bind and be washed away The OD values would be nearly the same as the assay control Both (a) and (b) Anti-human Ig-conjugate would bind non-specifically to the ELISA plate ANSWER DOWNLOAD EXAMIANS APP
Immunological Techniques Which of the following is a positively charged matrix for ion exchange chromatography? Phosphocellulose None of these DEAE cellulose CM cellulose Phosphocellulose None of these DEAE cellulose CM cellulose ANSWER DOWNLOAD EXAMIANS APP
Immunological Techniques The IPTG is a gratuitous inducers of (β-galactosidase, and x-gal is a chromogenic substrate. When α-complementation occurs which colour is produced? White Blue No colour is produced Brown White Blue No colour is produced Brown ANSWER DOWNLOAD EXAMIANS APP
Immunological Techniques Which of the following is an immunodiffusion test? All of these Gel diffusion Double-diffusion Ouchterloney technique All of these Gel diffusion Double-diffusion Ouchterloney technique ANSWER DOWNLOAD EXAMIANS APP