Immunological Techniques
In Counter immunoelectrophoresis

electrophoresis will drive the antibody and antigen parallel to each other
the antibody will migrate towards cathode
the antibody will migrate towards anode
electrophoresis will drive the antibody and antigen toward each other

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Immunological Techniques
What would happen if the anti-human Ig-conjugate is not washed free of the well before the substrate is added?

The ELISA would develop normally
None of these
The ELISA would not develop when the substrate was added
All wells would show uniform over-development due to unbound and excess anti-human Ig enzyme conjugate

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Immunological Techniques
What would happen if serum is omitted from the ELISA, but all other steps remain same?

Anti-human Ig-conjugate would bind non-specifically to the ELISA plate
Both (a) and (b)
Anti-human Ig-conjugate would not bind and be washed away
The OD values would be nearly the same as the assay control

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