Immunological Techniques Which of the following radioactive element is generally involved in RIA? Iodine-125 All of these Tritium Carbon-14 Iodine-125 All of these Tritium Carbon-14 ANSWER DOWNLOAD EXAMIANS APP
Immunological Techniques Which of the following is an immunodiffusion test? Ouchterloney technique Double-diffusion Gel diffusion All of these Ouchterloney technique Double-diffusion Gel diffusion All of these ANSWER DOWNLOAD EXAMIANS APP
Immunological Techniques What is the ELISA test intended to measure? Presence of free, circulating virus in the patient Antigen to HIV only Antibody to HIV only Antibodies directed against HLA molecules Presence of free, circulating virus in the patient Antigen to HIV only Antibody to HIV only Antibodies directed against HLA molecules ANSWER DOWNLOAD EXAMIANS APP
Immunological Techniques What would happen if serum is omitted from the ELISA, but all other steps remain same? Both (a) and (b) The OD values would be nearly the same as the assay control Anti-human Ig-conjugate would bind non-specifically to the ELISA plate Anti-human Ig-conjugate would not bind and be washed away Both (a) and (b) The OD values would be nearly the same as the assay control Anti-human Ig-conjugate would bind non-specifically to the ELISA plate Anti-human Ig-conjugate would not bind and be washed away ANSWER DOWNLOAD EXAMIANS APP
Immunological Techniques What would happen if the anti-human Ig-conjugate is not washed free of the well before the substrate is added? The ELISA would develop normally All wells would show uniform over-development due to unbound and excess anti-human Ig enzyme conjugate None of these The ELISA would not develop when the substrate was added The ELISA would develop normally All wells would show uniform over-development due to unbound and excess anti-human Ig enzyme conjugate None of these The ELISA would not develop when the substrate was added ANSWER DOWNLOAD EXAMIANS APP
Immunological Techniques An antigen preparation and an antibody preparation are tested by immunodiffusion. Three bands are found, indicating that there was one antibody and one antigen the antibody was impure the temperature was too high there was more than one antigen there was one antibody and one antigen the antibody was impure the temperature was too high there was more than one antigen ANSWER DOWNLOAD EXAMIANS APP