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Immunological Techniques

Immunological Techniques
Which of the following radioactive element is generally involved in RIA?

Tritium
All of these
Iodine-125
Carbon-14

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Immunological Techniques
Immunoaffinity chromatography can be used in biochemical applications to

break down antigen and analyze quantitatively
None of these
purify protein antigen
break down antibody structure

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Immunological Techniques
The competitive immunoassay can be used

to detect antibody associated with allergies (IgE)
both (a) and (b)
commonly to detect trace amounts of drugs.
to detect very small amounts of antigen

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Immunological Techniques
What radioactive molecule is used to end-label an oligonucleotide?

Any of these
(alpha)-32P-dUTP
(gamma)-32P-ATP
(alpha)-32P-ATP

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Immunological Techniques
DNA- footprinting is a suitable technique for identifying which of the following?

Protein binding site within DNA
Particular mRNA in mixture
Particular t-RNA in mixture
Introns within DNA

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Immunological Techniques
The IPTG is a gratuitous inducers of (β-galactosidase, and x-gal is a chromogenic substrate. When α-complementation occurs which colour is produced?

White
Blue
Brown
No colour is produced

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