Immunological Techniques
Immunoelectrophoresis techniques are designed to separate the mixture components from each other by using electrophoresis

after reaction with antibody
prior to reaction with antibody
after reaction with antigen
prior to reaction with antigen

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Immunological Techniques
Different form of a plasmid, on an agarose gel, after electrophoresis may be in which of the following sequence in decreasing molecular weight?

Open circular, super coiled, linear plasmid DNA
Open circular, super coiled, linear plasmid DNA
Linear plasmid, open circular, super coiled plasmid
Open circular, linear plasmid, super coiled plasmid

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Immunological Techniques
What would happen if the anti-human Ig-conjugate is not washed free of the well before the substrate is added?

The ELISA would not develop when the substrate was added
All wells would show uniform over-development due to unbound and excess anti-human Ig enzyme conjugate
None of these
The ELISA would develop normally

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