Immunological Techniques
Immunoelectrophoresis techniques are designed to separate the mixture components from each other by using electrophoresis

after reaction with antibody
prior to reaction with antigen
prior to reaction with antibody
after reaction with antigen

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Immunological Techniques
What would happen if the anti-human Ig-conjugate is not washed free of the well before the substrate is added?

The ELISA would develop normally
All wells would show uniform over-development due to unbound and excess anti-human Ig enzyme conjugate
None of these
The ELISA would not develop when the substrate was added

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Immunological Techniques
Different form of a plasmid, on an agarose gel, after electrophoresis may be in which of the following sequence in decreasing molecular weight?

Open circular, super coiled, linear plasmid DNA
Linear plasmid, open circular, super coiled plasmid
Open circular, linear plasmid, super coiled plasmid
Open circular, super coiled, linear plasmid DNA

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Immunological Techniques
What would happen if serum is omitted from the ELISA, but all other steps remain same?

Anti-human Ig-conjugate would not bind and be washed away
The OD values would be nearly the same as the assay control
Anti-human Ig-conjugate would bind non-specifically to the ELISA plate
Both (a) and (b)

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