Immunological Techniques
Immunoelectrophoresis techniques are designed to separate the mixture components from each other by using electrophoresis

prior to reaction with antigen
prior to reaction with antibody
after reaction with antibody
after reaction with antigen

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Immunological Techniques
What would happen if serum is omitted from the ELISA, but all other steps remain same?

Anti-human Ig-conjugate would not bind and be washed away
The OD values would be nearly the same as the assay control
Anti-human Ig-conjugate would bind non-specifically to the ELISA plate
Both (a) and (b)

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