Immunological Techniques
Immunoelectrophoresis techniques are designed to separate the mixture components from each other by using electrophoresis

prior to reaction with antibody
prior to reaction with antigen
after reaction with antibody
after reaction with antigen

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Immunological Techniques
What would happen if the anti-human Ig-conjugate is not washed free of the well before the substrate is added?

All wells would show uniform over-development due to unbound and excess anti-human Ig enzyme conjugate
The ELISA would develop normally
None of these
The ELISA would not develop when the substrate was added

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