Immunological Techniques What would happen if serum is omitted from the ELISA, but all other steps remain same? Anti-human Ig-conjugate would not bind and be washed away Both (a) and (b) The OD values would be nearly the same as the assay control Anti-human Ig-conjugate would bind non-specifically to the ELISA plate Anti-human Ig-conjugate would not bind and be washed away Both (a) and (b) The OD values would be nearly the same as the assay control Anti-human Ig-conjugate would bind non-specifically to the ELISA plate ANSWER DOWNLOAD EXAMIANS APP
Immunological Techniques What is approximatively the half life of 32-Phosphorus? 28 days 30 days 14 days 42 days 28 days 30 days 14 days 42 days ANSWER DOWNLOAD EXAMIANS APP
Immunological Techniques The IPTG is a gratuitous inducers of (β-galactosidase, and x-gal is a chromogenic substrate. When α-complementation occurs which colour is produced? No colour is produced Brown White Blue No colour is produced Brown White Blue ANSWER DOWNLOAD EXAMIANS APP
Immunological Techniques What is the ELISA test intended to measure? Presence of free, circulating virus in the patient Antigen to HIV only Antibody to HIV only Antibodies directed against HLA molecules Presence of free, circulating virus in the patient Antigen to HIV only Antibody to HIV only Antibodies directed against HLA molecules ANSWER DOWNLOAD EXAMIANS APP
Immunological Techniques RAST test (radio allergosorbent test) is often used to detect bacteriophages antibodies associated with allergies (IgE) None of these antigen associated with allergies (IgE) bacteriophages antibodies associated with allergies (IgE) None of these antigen associated with allergies (IgE) ANSWER DOWNLOAD EXAMIANS APP
Immunological Techniques What would happen if the anti-human Ig-conjugate is not washed free of the well before the substrate is added? None of these All wells would show uniform over-development due to unbound and excess anti-human Ig enzyme conjugate The ELISA would not develop when the substrate was added The ELISA would develop normally None of these All wells would show uniform over-development due to unbound and excess anti-human Ig enzyme conjugate The ELISA would not develop when the substrate was added The ELISA would develop normally ANSWER DOWNLOAD EXAMIANS APP
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