Immunological Techniques
What would happen if serum is omitted from the ELISA, but all other steps remain same?

Both (a) and (b)
Anti-human Ig-conjugate would not bind and be washed away
The OD values would be nearly the same as the assay control
Anti-human Ig-conjugate would bind non-specifically to the ELISA plate

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Immunological Techniques
In Counter immunoelectrophoresis

electrophoresis will drive the antibody and antigen parallel to each other
the antibody will migrate towards anode
electrophoresis will drive the antibody and antigen toward each other
the antibody will migrate towards cathode

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