Immunological Techniques What would happen if serum is omitted from the ELISA, but all other steps remain same? Both (a) and (b) Anti-human Ig-conjugate would bind non-specifically to the ELISA plate Anti-human Ig-conjugate would not bind and be washed away The OD values would be nearly the same as the assay control Both (a) and (b) Anti-human Ig-conjugate would bind non-specifically to the ELISA plate Anti-human Ig-conjugate would not bind and be washed away The OD values would be nearly the same as the assay control ANSWER DOWNLOAD EXAMIANS APP
Immunological Techniques Immunoaffinity chromatography can be used in biochemical applications to purify protein antigen break down antibody structure None of these break down antigen and analyze quantitatively purify protein antigen break down antibody structure None of these break down antigen and analyze quantitatively ANSWER DOWNLOAD EXAMIANS APP
Immunological Techniques What would happen if the anti-human Ig-conjugate is not washed free of the well before the substrate is added? The ELISA would develop normally All wells would show uniform over-development due to unbound and excess anti-human Ig enzyme conjugate None of these The ELISA would not develop when the substrate was added The ELISA would develop normally All wells would show uniform over-development due to unbound and excess anti-human Ig enzyme conjugate None of these The ELISA would not develop when the substrate was added ANSWER DOWNLOAD EXAMIANS APP
Immunological Techniques What is approximatively the half life of 32-Phosphorus? 14 days 28 days 42 days 30 days 14 days 28 days 42 days 30 days ANSWER DOWNLOAD EXAMIANS APP
Immunological Techniques What radioactive molecule is used to end-label an oligonucleotide? (alpha)-32P-ATP (gamma)-32P-ATP Any of these (alpha)-32P-dUTP (alpha)-32P-ATP (gamma)-32P-ATP Any of these (alpha)-32P-dUTP ANSWER DOWNLOAD EXAMIANS APP
Immunological Techniques What is the ELISA test intended to measure? Presence of free, circulating virus in the patient Antigen to HIV only Antibody to HIV only Antibodies directed against HLA molecules Presence of free, circulating virus in the patient Antigen to HIV only Antibody to HIV only Antibodies directed against HLA molecules ANSWER DOWNLOAD EXAMIANS APP
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