Immunological Techniques
What would happen if serum is omitted from the ELISA, but all other steps remain same?

The OD values would be nearly the same as the assay control
Anti-human Ig-conjugate would bind non-specifically to the ELISA plate
Both (a) and (b)
Anti-human Ig-conjugate would not bind and be washed away

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Immunological Techniques
In Counter immunoelectrophoresis

electrophoresis will drive the antibody and antigen parallel to each other
electrophoresis will drive the antibody and antigen toward each other
the antibody will migrate towards cathode
the antibody will migrate towards anode

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Immunological Techniques
In Rocket Immunodiffusion the length of the rocket is

inversely proportional to the amount of antigen placed in each well
inversely proportional to the amount of antibody placed in each well
proportional to the amount of antibody placed in each well
proportional to the amount of antigen placed in each well

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