Immunological Techniques
What would happen if serum is omitted from the ELISA, but all other steps remain same?

Anti-human Ig-conjugate would not bind and be washed away
The OD values would be nearly the same as the assay control
Both (a) and (b)
Anti-human Ig-conjugate would bind non-specifically to the ELISA plate

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Immunological Techniques
In Rocket Immunodiffusion the length of the rocket is

inversely proportional to the amount of antibody placed in each well
proportional to the amount of antigen placed in each well
proportional to the amount of antibody placed in each well
inversely proportional to the amount of antigen placed in each well

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