Immunological Techniques What would happen if serum is omitted from the ELISA, but all other steps remain same? Anti-human Ig-conjugate would bind non-specifically to the ELISA plate Both (a) and (b) Anti-human Ig-conjugate would not bind and be washed away The OD values would be nearly the same as the assay control Anti-human Ig-conjugate would bind non-specifically to the ELISA plate Both (a) and (b) Anti-human Ig-conjugate would not bind and be washed away The OD values would be nearly the same as the assay control ANSWER DOWNLOAD EXAMIANS APP
Immunological Techniques What would happen if the anti-human Ig-conjugate is not washed free of the well before the substrate is added? None of these All wells would show uniform over-development due to unbound and excess anti-human Ig enzyme conjugate The ELISA would not develop when the substrate was added The ELISA would develop normally None of these All wells would show uniform over-development due to unbound and excess anti-human Ig enzyme conjugate The ELISA would not develop when the substrate was added The ELISA would develop normally ANSWER DOWNLOAD EXAMIANS APP
Immunological Techniques DNA- footprinting is a suitable technique for identifying which of the following? Introns within DNA Protein binding site within DNA Particular t-RNA in mixture Particular mRNA in mixture Introns within DNA Protein binding site within DNA Particular t-RNA in mixture Particular mRNA in mixture ANSWER DOWNLOAD EXAMIANS APP
Immunological Techniques Immunoelectrophoresis techniques are designed to separate the mixture components from each other by using electrophoresis after reaction with antigen prior to reaction with antigen prior to reaction with antibody after reaction with antibody after reaction with antigen prior to reaction with antigen prior to reaction with antibody after reaction with antibody ANSWER DOWNLOAD EXAMIANS APP
Immunological Techniques The competitive immunoassay can be used commonly to detect trace amounts of drugs. to detect antibody associated with allergies (IgE) both (a) and (b) to detect very small amounts of antigen commonly to detect trace amounts of drugs. to detect antibody associated with allergies (IgE) both (a) and (b) to detect very small amounts of antigen ANSWER DOWNLOAD EXAMIANS APP
Immunological Techniques Immunoaffinity chromatography can be used in biochemical applications to purify protein antigen None of these break down antigen and analyze quantitatively break down antibody structure purify protein antigen None of these break down antigen and analyze quantitatively break down antibody structure ANSWER DOWNLOAD EXAMIANS APP
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