Immunological Techniques What would happen if the anti-human Ig-conjugate is not washed free of the well before the substrate is added? All wells would show uniform over-development due to unbound and excess anti-human Ig enzyme conjugate None of these The ELISA would develop normally The ELISA would not develop when the substrate was added All wells would show uniform over-development due to unbound and excess anti-human Ig enzyme conjugate None of these The ELISA would develop normally The ELISA would not develop when the substrate was added ANSWER DOWNLOAD EXAMIANS APP
Immunological Techniques The IPTG is a gratuitous inducers of (β-galactosidase, and x-gal is a chromogenic substrate. When α-complementation occurs which colour is produced? White Blue No colour is produced Brown White Blue No colour is produced Brown ANSWER DOWNLOAD EXAMIANS APP
Immunological Techniques What would happen if serum is omitted from the ELISA, but all other steps remain same? Anti-human Ig-conjugate would not bind and be washed away Both (a) and (b) The OD values would be nearly the same as the assay control Anti-human Ig-conjugate would bind non-specifically to the ELISA plate Anti-human Ig-conjugate would not bind and be washed away Both (a) and (b) The OD values would be nearly the same as the assay control Anti-human Ig-conjugate would bind non-specifically to the ELISA plate ANSWER DOWNLOAD EXAMIANS APP
Immunological Techniques Radial Immunodiffusion is similar to double-diffusion gel diffusion all of these Ouchterloney technique double-diffusion gel diffusion all of these Ouchterloney technique ANSWER DOWNLOAD EXAMIANS APP
Immunological Techniques Which of the following is a positively charged matrix for ion exchange chromatography? CM cellulose DEAE cellulose Phosphocellulose None of these CM cellulose DEAE cellulose Phosphocellulose None of these ANSWER DOWNLOAD EXAMIANS APP
Immunological Techniques Immunoelectrophoresis techniques are designed to separate the mixture components from each other by using electrophoresis prior to reaction with antigen after reaction with antigen prior to reaction with antibody after reaction with antibody prior to reaction with antigen after reaction with antigen prior to reaction with antibody after reaction with antibody ANSWER DOWNLOAD EXAMIANS APP
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