Immunological Techniques
What would happen if the anti-human Ig-conjugate is not washed free of the well before the substrate is added?

None of these
All wells would show uniform over-development due to unbound and excess anti-human Ig enzyme conjugate
The ELISA would not develop when the substrate was added
The ELISA would develop normally

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Immunological Techniques
In Rocket Immunodiffusion the length of the rocket is

inversely proportional to the amount of antibody placed in each well
inversely proportional to the amount of antigen placed in each well
proportional to the amount of antigen placed in each well
proportional to the amount of antibody placed in each well

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