Immunological Techniques What would happen if the anti-human Ig-conjugate is not washed free of the well before the substrate is added? None of these The ELISA would develop normally All wells would show uniform over-development due to unbound and excess anti-human Ig enzyme conjugate The ELISA would not develop when the substrate was added None of these The ELISA would develop normally All wells would show uniform over-development due to unbound and excess anti-human Ig enzyme conjugate The ELISA would not develop when the substrate was added ANSWER DOWNLOAD EXAMIANS APP
Immunological Techniques What would happen if serum is omitted from the ELISA, but all other steps remain same? The OD values would be nearly the same as the assay control Both (a) and (b) Anti-human Ig-conjugate would bind non-specifically to the ELISA plate Anti-human Ig-conjugate would not bind and be washed away The OD values would be nearly the same as the assay control Both (a) and (b) Anti-human Ig-conjugate would bind non-specifically to the ELISA plate Anti-human Ig-conjugate would not bind and be washed away ANSWER DOWNLOAD EXAMIANS APP
Immunological Techniques Immunoaffinity chromatography can be used in biochemical applications to purify protein antigen None of these break down antibody structure break down antigen and analyze quantitatively purify protein antigen None of these break down antibody structure break down antigen and analyze quantitatively ANSWER DOWNLOAD EXAMIANS APP
Immunological Techniques RAST test (radio allergosorbent test) is often used to detect bacteriophages antigen associated with allergies (IgE) None of these antibodies associated with allergies (IgE) bacteriophages antigen associated with allergies (IgE) None of these antibodies associated with allergies (IgE) ANSWER DOWNLOAD EXAMIANS APP
Immunological Techniques What is the ELISA test intended to measure? Presence of free, circulating virus in the patient Antigen to HIV only Antibodies directed against HLA molecules Antibody to HIV only Presence of free, circulating virus in the patient Antigen to HIV only Antibodies directed against HLA molecules Antibody to HIV only ANSWER DOWNLOAD EXAMIANS APP
Immunological Techniques The IPTG is a gratuitous inducers of (β-galactosidase, and x-gal is a chromogenic substrate. When α-complementation occurs which colour is produced? No colour is produced White Blue Brown No colour is produced White Blue Brown ANSWER DOWNLOAD EXAMIANS APP
EXAMIANS