Immunological Techniques What would happen if the anti-human Ig-conjugate is not washed free of the well before the substrate is added? All wells would show uniform over-development due to unbound and excess anti-human Ig enzyme conjugate The ELISA would develop normally The ELISA would not develop when the substrate was added None of these All wells would show uniform over-development due to unbound and excess anti-human Ig enzyme conjugate The ELISA would develop normally The ELISA would not develop when the substrate was added None of these ANSWER DOWNLOAD EXAMIANS APP
Immunological Techniques What radioactive molecule is used to end-label an oligonucleotide? (alpha)-32P-dUTP (alpha)-32P-ATP (gamma)-32P-ATP Any of these (alpha)-32P-dUTP (alpha)-32P-ATP (gamma)-32P-ATP Any of these ANSWER DOWNLOAD EXAMIANS APP
Immunological Techniques DNA- footprinting is a suitable technique for identifying which of the following? Protein binding site within DNA Introns within DNA Particular mRNA in mixture Particular t-RNA in mixture Protein binding site within DNA Introns within DNA Particular mRNA in mixture Particular t-RNA in mixture ANSWER DOWNLOAD EXAMIANS APP
Immunological Techniques Different form of a plasmid, on an agarose gel, after electrophoresis may be in which of the following sequence in decreasing molecular weight? Linear plasmid, open circular, super coiled plasmid Open circular, linear plasmid, super coiled plasmid Open circular, super coiled, linear plasmid DNA Open circular, super coiled, linear plasmid DNA Linear plasmid, open circular, super coiled plasmid Open circular, linear plasmid, super coiled plasmid Open circular, super coiled, linear plasmid DNA Open circular, super coiled, linear plasmid DNA ANSWER DOWNLOAD EXAMIANS APP
Immunological Techniques An antigen preparation and an antibody preparation are tested by immunodiffusion. Three bands are found, indicating that the temperature was too high there was one antibody and one antigen there was more than one antigen the antibody was impure the temperature was too high there was one antibody and one antigen there was more than one antigen the antibody was impure ANSWER DOWNLOAD EXAMIANS APP
Immunological Techniques Which of the following is an immunodiffusion test? Double-diffusion Gel diffusion All of these Ouchterloney technique Double-diffusion Gel diffusion All of these Ouchterloney technique ANSWER DOWNLOAD EXAMIANS APP