Immunological Techniques What would happen if the anti-human Ig-conjugate is not washed free of the well before the substrate is added? The ELISA would not develop when the substrate was added None of these All wells would show uniform over-development due to unbound and excess anti-human Ig enzyme conjugate The ELISA would develop normally The ELISA would not develop when the substrate was added None of these All wells would show uniform over-development due to unbound and excess anti-human Ig enzyme conjugate The ELISA would develop normally ANSWER DOWNLOAD EXAMIANS APP
Immunological Techniques An antigen preparation and an antibody preparation are tested by immunodiffusion. Three bands are found, indicating that the antibody was impure the temperature was too high there was one antibody and one antigen there was more than one antigen the antibody was impure the temperature was too high there was one antibody and one antigen there was more than one antigen ANSWER DOWNLOAD EXAMIANS APP
Immunological Techniques Agarose gel electrophoresis and pulsed field gel electrophoresis may be used to resolve respectively 1000 kb and l0kb DNA 20 kb and 2000 kb DNA 10 kb and 1000 kb DNA 2000 kb and 20kb DNA 1000 kb and l0kb DNA 20 kb and 2000 kb DNA 10 kb and 1000 kb DNA 2000 kb and 20kb DNA ANSWER DOWNLOAD EXAMIANS APP
Immunological Techniques What would happen if serum is omitted from the ELISA, but all other steps remain same? Anti-human Ig-conjugate would bind non-specifically to the ELISA plate Anti-human Ig-conjugate would not bind and be washed away The OD values would be nearly the same as the assay control Both (a) and (b) Anti-human Ig-conjugate would bind non-specifically to the ELISA plate Anti-human Ig-conjugate would not bind and be washed away The OD values would be nearly the same as the assay control Both (a) and (b) ANSWER DOWNLOAD EXAMIANS APP
Immunological Techniques Which of the following is an immunodiffusion test? All of these Ouchterloney technique Gel diffusion Double-diffusion All of these Ouchterloney technique Gel diffusion Double-diffusion ANSWER DOWNLOAD EXAMIANS APP
Immunological Techniques Which of the following is a positively charged matrix for ion exchange chromatography? CM cellulose Phosphocellulose None of these DEAE cellulose CM cellulose Phosphocellulose None of these DEAE cellulose ANSWER DOWNLOAD EXAMIANS APP
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