Immunological Techniques What would happen if the anti-human Ig-conjugate is not washed free of the well before the substrate is added? The ELISA would not develop when the substrate was added The ELISA would develop normally All wells would show uniform over-development due to unbound and excess anti-human Ig enzyme conjugate None of these The ELISA would not develop when the substrate was added The ELISA would develop normally All wells would show uniform over-development due to unbound and excess anti-human Ig enzyme conjugate None of these ANSWER DOWNLOAD EXAMIANS APP
Immunological Techniques Immunoaffinity chromatography can be used in biochemical applications to None of these break down antigen and analyze quantitatively break down antibody structure purify protein antigen None of these break down antigen and analyze quantitatively break down antibody structure purify protein antigen ANSWER DOWNLOAD EXAMIANS APP
Immunological Techniques What radioactive molecule is used to end-label an oligonucleotide? (alpha)-32P-dUTP (gamma)-32P-ATP Any of these (alpha)-32P-ATP (alpha)-32P-dUTP (gamma)-32P-ATP Any of these (alpha)-32P-ATP ANSWER DOWNLOAD EXAMIANS APP
Immunological Techniques An antigen preparation and an antibody preparation are tested by immunodiffusion. Three bands are found, indicating that the temperature was too high there was more than one antigen the antibody was impure there was one antibody and one antigen the temperature was too high there was more than one antigen the antibody was impure there was one antibody and one antigen ANSWER DOWNLOAD EXAMIANS APP
Immunological Techniques RAST test (radio allergosorbent test) is often used to detect antibodies associated with allergies (IgE) antigen associated with allergies (IgE) None of these bacteriophages antibodies associated with allergies (IgE) antigen associated with allergies (IgE) None of these bacteriophages ANSWER DOWNLOAD EXAMIANS APP
Immunological Techniques What is the ELISA test intended to measure? Presence of free, circulating virus in the patient Antibody to HIV only Antigen to HIV only Antibodies directed against HLA molecules Presence of free, circulating virus in the patient Antibody to HIV only Antigen to HIV only Antibodies directed against HLA molecules ANSWER DOWNLOAD EXAMIANS APP