Immunological Techniques
What would happen if the anti-human Ig-conjugate is not washed free of the well before the substrate is added?

The ELISA would develop normally
None of these
The ELISA would not develop when the substrate was added
All wells would show uniform over-development due to unbound and excess anti-human Ig enzyme conjugate

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Immunological Techniques
Different form of a plasmid, on an agarose gel, after electrophoresis may be in which of the following sequence in decreasing molecular weight?

Open circular, super coiled, linear plasmid DNA
Open circular, linear plasmid, super coiled plasmid
Open circular, super coiled, linear plasmid DNA
Linear plasmid, open circular, super coiled plasmid

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Immunological Techniques
In Counter immunoelectrophoresis

electrophoresis will drive the antibody and antigen toward each other
the antibody will migrate towards cathode
electrophoresis will drive the antibody and antigen parallel to each other
the antibody will migrate towards anode

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