Immunological Techniques What would happen if the anti-human Ig-conjugate is not washed free of the well before the substrate is added? The ELISA would not develop when the substrate was added The ELISA would develop normally All wells would show uniform over-development due to unbound and excess anti-human Ig enzyme conjugate None of these The ELISA would not develop when the substrate was added The ELISA would develop normally All wells would show uniform over-development due to unbound and excess anti-human Ig enzyme conjugate None of these ANSWER DOWNLOAD EXAMIANS APP
Immunological Techniques What radioactive molecule is used to end-label an oligonucleotide? (alpha)-32P-ATP (gamma)-32P-ATP Any of these (alpha)-32P-dUTP (alpha)-32P-ATP (gamma)-32P-ATP Any of these (alpha)-32P-dUTP ANSWER DOWNLOAD EXAMIANS APP
Immunological Techniques Immunoaffinity chromatography can be used in biochemical applications to None of these purify protein antigen break down antibody structure break down antigen and analyze quantitatively None of these purify protein antigen break down antibody structure break down antigen and analyze quantitatively ANSWER DOWNLOAD EXAMIANS APP
Immunological Techniques The competitive immunoassay can be used both (a) and (b) to detect very small amounts of antigen commonly to detect trace amounts of drugs. to detect antibody associated with allergies (IgE) both (a) and (b) to detect very small amounts of antigen commonly to detect trace amounts of drugs. to detect antibody associated with allergies (IgE) ANSWER DOWNLOAD EXAMIANS APP
Immunological Techniques Different form of a plasmid, on an agarose gel, after electrophoresis may be in which of the following sequence in decreasing molecular weight? Linear plasmid, open circular, super coiled plasmid Open circular, linear plasmid, super coiled plasmid Open circular, super coiled, linear plasmid DNA Open circular, super coiled, linear plasmid DNA Linear plasmid, open circular, super coiled plasmid Open circular, linear plasmid, super coiled plasmid Open circular, super coiled, linear plasmid DNA Open circular, super coiled, linear plasmid DNA ANSWER DOWNLOAD EXAMIANS APP
Immunological Techniques What is the ELISA test intended to measure? Antibodies directed against HLA molecules Antigen to HIV only Presence of free, circulating virus in the patient Antibody to HIV only Antibodies directed against HLA molecules Antigen to HIV only Presence of free, circulating virus in the patient Antibody to HIV only ANSWER DOWNLOAD EXAMIANS APP
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