Immunological Techniques
In Rocket Immunodiffusion the length of the rocket is

proportional to the amount of antibody placed in each well
inversely proportional to the amount of antibody placed in each well
inversely proportional to the amount of antigen placed in each well
proportional to the amount of antigen placed in each well

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Immunological Techniques
What would happen if serum is omitted from the ELISA, but all other steps remain same?

Anti-human Ig-conjugate would not bind and be washed away
Anti-human Ig-conjugate would bind non-specifically to the ELISA plate
Both (a) and (b)
The OD values would be nearly the same as the assay control

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Immunological Techniques
What would happen if the anti-human Ig-conjugate is not washed free of the well before the substrate is added?

The ELISA would not develop when the substrate was added
The ELISA would develop normally
All wells would show uniform over-development due to unbound and excess anti-human Ig enzyme conjugate
None of these

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