Immunological Techniques
What would happen if the anti-human Ig-conjugate is not washed free of the well before the substrate is added?

All wells would show uniform over-development due to unbound and excess anti-human Ig enzyme conjugate
The ELISA would not develop when the substrate was added
None of these
The ELISA would develop normally

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Immunological Techniques
In Counter immunoelectrophoresis

electrophoresis will drive the antibody and antigen toward each other
the antibody will migrate towards anode
electrophoresis will drive the antibody and antigen parallel to each other
the antibody will migrate towards cathode

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