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Immunological Techniques

Immunological Techniques
Agarose a porous matrix used in various molecular biology technique is made by repetitive units of

none of these
agarobiose
maltose
agar

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Immunological Techniques
Immunoaffinity chromatography can be used in biochemical applications to

break down antibody structure
None of these
break down antigen and analyze quantitatively
purify protein antigen

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Immunological Techniques
What is the ELISA test intended to measure?

Antibodies directed against HLA molecules
Antibody to HIV only
Antigen to HIV only
Presence of free, circulating virus in the patient

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Immunological Techniques
Which of the following is a positively charged matrix for ion exchange chromatography?

None of these
Phosphocellulose
CM cellulose
DEAE cellulose

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Immunological Techniques
What radioactive molecule is used to end-label an oligonucleotide?

Any of these
(gamma)-32P-ATP
(alpha)-32P-dUTP
(alpha)-32P-ATP

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Immunological Techniques
The IPTG is a gratuitous inducers of (β-galactosidase, and x-gal is a chromogenic substrate. When α-complementation occurs which colour is produced?

Blue
Brown
No colour is produced
White

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