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Immunological Techniques

Immunological Techniques
Agarose a porous matrix used in various molecular biology technique is made by repetitive units of

maltose
none of these
agar
agarobiose

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Immunological Techniques
DNA- footprinting is a suitable technique for identifying which of the following?

Protein binding site within DNA
Particular mRNA in mixture
Introns within DNA
Particular t-RNA in mixture

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Immunological Techniques
The competitive immunoassay can be used

to detect very small amounts of antigen
to detect antibody associated with allergies (IgE)
both (a) and (b)
commonly to detect trace amounts of drugs.

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Immunological Techniques
Agarose gel electrophoresis and pulsed field gel electrophoresis may be used to resolve respectively

2000 kb and 20kb DNA
20 kb and 2000 kb DNA
10 kb and 1000 kb DNA
1000 kb and l0kb DNA

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Immunological Techniques
What is the ELISA test intended to measure?

Antibodies directed against HLA molecules
Antigen to HIV only
Antibody to HIV only
Presence of free, circulating virus in the patient

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Immunological Techniques
Which of the following is a positively charged matrix for ion exchange chromatography?

None of these
DEAE cellulose
Phosphocellulose
CM cellulose

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