Immunological Techniques DNA- footprinting is a suitable technique for identifying which of the following? Protein binding site within DNA Particular mRNA in mixture Particular t-RNA in mixture Introns within DNA Protein binding site within DNA Particular mRNA in mixture Particular t-RNA in mixture Introns within DNA ANSWER DOWNLOAD EXAMIANS APP
Immunological Techniques What would happen if serum is omitted from the ELISA, but all other steps remain same? Both (a) and (b) Anti-human Ig-conjugate would bind non-specifically to the ELISA plate The OD values would be nearly the same as the assay control Anti-human Ig-conjugate would not bind and be washed away Both (a) and (b) Anti-human Ig-conjugate would bind non-specifically to the ELISA plate The OD values would be nearly the same as the assay control Anti-human Ig-conjugate would not bind and be washed away ANSWER DOWNLOAD EXAMIANS APP
Immunological Techniques RAST test (radio allergosorbent test) is often used to detect None of these antigen associated with allergies (IgE) bacteriophages antibodies associated with allergies (IgE) None of these antigen associated with allergies (IgE) bacteriophages antibodies associated with allergies (IgE) ANSWER DOWNLOAD EXAMIANS APP
Immunological Techniques What would happen if the anti-human Ig-conjugate is not washed free of the well before the substrate is added? The ELISA would not develop when the substrate was added None of these The ELISA would develop normally All wells would show uniform over-development due to unbound and excess anti-human Ig enzyme conjugate The ELISA would not develop when the substrate was added None of these The ELISA would develop normally All wells would show uniform over-development due to unbound and excess anti-human Ig enzyme conjugate ANSWER DOWNLOAD EXAMIANS APP
Immunological Techniques An antigen preparation and an antibody preparation are tested by immunodiffusion. Three bands are found, indicating that the antibody was impure there was more than one antigen the temperature was too high there was one antibody and one antigen the antibody was impure there was more than one antigen the temperature was too high there was one antibody and one antigen ANSWER DOWNLOAD EXAMIANS APP