Immunological Techniques DNA- footprinting is a suitable technique for identifying which of the following? Particular t-RNA in mixture Introns within DNA Particular mRNA in mixture Protein binding site within DNA Particular t-RNA in mixture Introns within DNA Particular mRNA in mixture Protein binding site within DNA ANSWER DOWNLOAD EXAMIANS APP
Immunological Techniques What would happen if serum is omitted from the ELISA, but all other steps remain same? Anti-human Ig-conjugate would bind non-specifically to the ELISA plate Both (a) and (b) The OD values would be nearly the same as the assay control Anti-human Ig-conjugate would not bind and be washed away Anti-human Ig-conjugate would bind non-specifically to the ELISA plate Both (a) and (b) The OD values would be nearly the same as the assay control Anti-human Ig-conjugate would not bind and be washed away ANSWER DOWNLOAD EXAMIANS APP
Immunological Techniques RAST test (radio allergosorbent test) is often used to detect antibodies associated with allergies (IgE) bacteriophages antigen associated with allergies (IgE) None of these antibodies associated with allergies (IgE) bacteriophages antigen associated with allergies (IgE) None of these ANSWER DOWNLOAD EXAMIANS APP
Immunological Techniques What would happen if the anti-human Ig-conjugate is not washed free of the well before the substrate is added? None of these The ELISA would not develop when the substrate was added All wells would show uniform over-development due to unbound and excess anti-human Ig enzyme conjugate The ELISA would develop normally None of these The ELISA would not develop when the substrate was added All wells would show uniform over-development due to unbound and excess anti-human Ig enzyme conjugate The ELISA would develop normally ANSWER DOWNLOAD EXAMIANS APP
Immunological Techniques An antigen preparation and an antibody preparation are tested by immunodiffusion. Three bands are found, indicating that the antibody was impure there was more than one antigen there was one antibody and one antigen the temperature was too high the antibody was impure there was more than one antigen there was one antibody and one antigen the temperature was too high ANSWER DOWNLOAD EXAMIANS APP