Immunological Techniques
What would happen if serum is omitted from the ELISA, but all other steps remain same?

Anti-human Ig-conjugate would bind non-specifically to the ELISA plate
The OD values would be nearly the same as the assay control
Both (a) and (b)
Anti-human Ig-conjugate would not bind and be washed away

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Immunological Techniques
What would happen if the anti-human Ig-conjugate is not washed free of the well before the substrate is added?

None of these
The ELISA would develop normally
All wells would show uniform over-development due to unbound and excess anti-human Ig enzyme conjugate
The ELISA would not develop when the substrate was added

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