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GENE TRANSFER IN PLANTS

Gene Transfer in Plants
T-DNA transfer and processing into plant genome requires products of which of the following genes?

vir D,E
vir A,B
vir G,C
All of these

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Gene Transfer in Plants
Because of large size of Ti-plasmid, intermediate vectors (IV) are developed in which T DNA has been subcloned into

pBR 322 based plasmid vector
all of these
pRK 2013
pRN 3

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Gene Transfer in Plants
The transfer of intermediate vectors into Agrobacterium are brought about by

triparental mating
biparental mating
transduction
transformation

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Gene Transfer in Plants
In response to the activating signal molecule, an endonuclease is produced that causes nicks in the T-DNA. It is encoded by

vir B
vir A
vir D
vir C

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Gene Transfer in Plants
Microinjection involves

All of these
injection of large amount of DNA
injection of DNA into bigger cells
injection with needle having diameter greater than cell diameter

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