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Gene Transfer in Plants

Gene Transfer in Plants
T-DNA transfer and processing into plant genome requires products of which of the following genes?

vir D,E
vir A,B
All of these
vir G,C

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Gene Transfer in Plants
Which of the following is not true about the direct repeats flanking T-DNA?

They are conserved between nopaline and octopine Ti-plasmids
These repeats are transferred intact to the plant genome
All of these
These are important in integration mechanism

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Gene Transfer in Plants
The right segment of octopine T-DNA (TR) is necessary for

tumour formation
enzymes for agropine biosynthesis
all of these
conjugative transfer

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Gene Transfer in Plants
Plant transformation vectors based on Agrobacterium can generally be divided into

six vectors
two vectors
four vectors
eight vectors

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Gene Transfer in Plants
Because of large size of Ti-plasmid, intermediate vectors (IV) are developed in which T DNA has been subcloned into

pBR 322 based plasmid vector
pRK 2013
all of these
pRN 3

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Gene Transfer in Plants
Intermediate vectors containing T-DNA are conjugation deficient. Thus conjugation is mediated in presence of which of the following plasmid?

pRN 3
pRK 2013
either (a) or (b)
none of these

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