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Gene Transfer in Plants

Gene Transfer in Plants
T-DNA transfer and processing into plant genome requires products of which of the following genes?

All of these
vir D,E
vir A,B
vir G,C

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Gene Transfer in Plants
Intermediate vectors containing T-DNA are conjugation deficient. Thus conjugation is mediated in presence of which of the following plasmid?

either (a) or (b)
pRK 2013
pRN 3
none of these

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Gene Transfer in Plants
Which of the following is not true about the helper plasmids?

These help in the mediating conjugation of intermediate vectors
These can't replicate in Agrobacterium
All of these
These can replicate in Agrobacterium

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Gene Transfer in Plants
Which of the following is not true about the direct repeats flanking T-DNA?

These are important in integration mechanism
They are conserved between nopaline and octopine Ti-plasmids
All of these
These repeats are transferred intact to the plant genome

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Gene Transfer in Plants
Opine synthesis is the property

conferred to plant cells when it transformed by Agrobacterium tumefaciens
of normal plant cells
determined by the bacteria Agrobacterium tumefaciens
both (a) and (b)

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Gene Transfer in Plants
The direct repeats flanking the T-DNA of Agrobacterium tume-faciens are known as

border sequences
cos site
transfer sequences
flanking sequences

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