Gene Transfer in Plants
Crown gall tissue

can be cultivated in vitro in absence of bacteria
both (a) and (b)
shows tumorous properties only in presence of bacteria
retains tumorous properties when cultivated

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Gene Transfer in Plants
In a plant tumour cell

complete Ti plasmid is incorporated in plant nuclear DNA
only a small specific segment of callus T DNA is incorporated
may vary from plant to plant
different parts of the Ti-plasmid are incorporated

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Gene Transfer in Plants
Advantage of microprojectile method over microinjection method for gene transfer in plants include

method is universal in its application irrespective of all shape, size, type and presence or absence of cell wall
All of these
intact cells are used
gene can be transferred to many cells simultaneously

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