Gene Transfer in Plants
Crown gall tissue

can be cultivated in vitro in absence of bacteria
retains tumorous properties when cultivated
shows tumorous properties only in presence of bacteria
both (a) and (b)

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Gene Transfer in Plants
Virulent strains of Agrobacterium contain large Ti-plasmids, which are responsible for the DNA transfer and subsequent disease symptoms. It has been shown that Ti-plasmids contain

three sets of sequence necessary for gene transfer
four sets of sequence necessary for gene transfer
two sets of sequence necessary for gene transfer
one set of sequence necessary for gene transfer

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Gene Transfer in Plants
Which of the following is true about T DNA?

Integration of T DNA occurs only at one specific sites in the plant nuclear DNA
Integration of T DNA occurs at two specific sites in the plant nuclear DNA
Integration of T DNA can occur at many different, apparently random, sites in the plant nuclear DNA
Integration of T DNA occurs at one site that may be random in the plant nuclear DNA

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