Gene Transfer in Plants
Crown gall tissue

both (a) and (b)
retains tumorous properties when cultivated
can be cultivated in vitro in absence of bacteria
shows tumorous properties only in presence of bacteria

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Gene Transfer in Plants
Advantage of microprojectile method over microinjection method for gene transfer in plants include

All of these
method is universal in its application irrespective of all shape, size, type and presence or absence of cell wall
intact cells are used
gene can be transferred to many cells simultaneously

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Gene Transfer in Plants
In a plant tumour cell

different parts of the Ti-plasmid are incorporated
only a small specific segment of callus T DNA is incorporated
complete Ti plasmid is incorporated in plant nuclear DNA
may vary from plant to plant

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