Gene Transfer in Plants
Virulent strains of Agrobacterium contain large Ti-plasmids, which are responsible for the DNA transfer and subsequent disease symptoms. It has been shown that Ti-plasmids contain

two sets of sequence necessary for gene transfer
one set of sequence necessary for gene transfer
four sets of sequence necessary for gene transfer
three sets of sequence necessary for gene transfer

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Gene Transfer in Plants
Which of the following is not true for microinjection technique that involves transfer of DNA into protoplast?

It employs needle with diameter greater than cell diameter
It is carried out with the help of micromanipulator
All of these
The recipient cells are immobilized on artificial support or artificially bound to substarate

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Gene Transfer in Plants
Crown gall tissue

can be cultivated in vitro in absence of bacteria
shows tumorous properties only in presence of bacteria
both (a) and (b)
retains tumorous properties when cultivated

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