Gene Transfer in Plants
Virulent strains of Agrobacterium contain large Ti-plasmids, which are responsible for the DNA transfer and subsequent disease symptoms. It has been shown that Ti-plasmids contain

one set of sequence necessary for gene transfer
four sets of sequence necessary for gene transfer
three sets of sequence necessary for gene transfer
two sets of sequence necessary for gene transfer

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Gene Transfer in Plants
Which of the following is not true for microinjection technique that involves transfer of DNA into protoplast?

It is carried out with the help of micromanipulator
All of these
The recipient cells are immobilized on artificial support or artificially bound to substarate
It employs needle with diameter greater than cell diameter

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