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Gene Transfer in Plants

Gene Transfer in Plants
The transfer of intermediate vectors into Agrobacterium are brought about by

biparental mating
triparental mating
transformation
transduction

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Gene Transfer in Plants
On Ti-plasmid T-region or T-DNA is flanked by a direct repeat of

25 bp
12 bp
20 bp
30 bp

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Gene Transfer in Plants
Microinjection involves

injection of large amount of DNA
All of these
injection of DNA into bigger cells
injection with needle having diameter greater than cell diameter

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Gene Transfer in Plants
Because of large size of Ti-plasmid, intermediate vectors (IV) are developed in which T DNA has been subcloned into

pRK 2013
pBR 322 based plasmid vector
all of these
pRN 3

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Gene Transfer in Plants
Integrated octopine T DNA occurs as

single segment
four segments
two segments
three segments

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Gene Transfer in Plants
Which of the following is true about Agrobacterium tumefaciens?

All of these
It infects dicotyledonous angiosperms
It infects gymosperms
It causes crown gall disease of plants

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