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Gene Transfer in Plants

Gene Transfer in Plants
The transfer of intermediate vectors into Agrobacterium are brought about by

triparental mating
transformation
transduction
biparental mating

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Gene Transfer in Plants
Direct DNA uptake by protoplasts can be stimulated by

decanal
polyethylene glycol (PEG)
luciferin
all of these

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Gene Transfer in Plants
Microinjection involves

All of these
injection with needle having diameter greater than cell diameter
injection of DNA into bigger cells
injection of large amount of DNA

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Gene Transfer in Plants
Integrated octopine T DNA occurs as

two segments
four segments
three segments
single segment

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Gene Transfer in Plants
The right segment of octopine T-DNA (TR) is necessary for

conjugative transfer
all of these
tumour formation
enzymes for agropine biosynthesis

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Gene Transfer in Plants
Intermediate vectors containing T-DNA are conjugation deficient. Thus conjugation is mediated in presence of which of the following plasmid?

pRN 3
either (a) or (b)
none of these
pRK 2013

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