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Gene Transfer in Plants

Gene Transfer in Plants
The transfer of intermediate vectors into Agrobacterium are brought about by

triparental mating
transduction
biparental mating
transformation

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Gene Transfer in Plants
Intermediate vectors containing T-DNA are conjugation deficient. Thus conjugation is mediated in presence of which of the following plasmid?

pRN 3
pRK 2013
none of these
either (a) or (b)

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Gene Transfer in Plants
Which of the following is not true about the helper plasmids?

These can't replicate in Agrobacterium
All of these
These help in the mediating conjugation of intermediate vectors
These can replicate in Agrobacterium

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Gene Transfer in Plants
Liposomes mediated gene transfer in plants involves

plasmid DNA enclosed in a lipid bag
fusion of liposomes with protoplast
use of polyethylene glycol (PEG)
All of these

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Gene Transfer in Plants
Because of large size of Ti-plasmid, intermediate vectors (IV) are developed in which T DNA has been subcloned into

all of these
pRN 3
pRK 2013
pBR 322 based plasmid vector

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Gene Transfer in Plants
Opine synthesis is the property

of normal plant cells
both (a) and (b)
determined by the bacteria Agrobacterium tumefaciens
conferred to plant cells when it transformed by Agrobacterium tumefaciens

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