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Gene Transfer in Plants

Gene Transfer in Plants
The transfer of intermediate vectors into Agrobacterium are brought about by

biparental mating
triparental mating
transformation
transduction

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Gene Transfer in Plants
Which of the following is not true about the helper plasmids?

These can't replicate in Agrobacterium
These help in the mediating conjugation of intermediate vectors
These can replicate in Agrobacterium
All of these

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Gene Transfer in Plants
Integrated octopine T DNA occurs as

single segment
four segments
two segments
three segments

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Gene Transfer in Plants
Opine synthesis is the property

conferred to plant cells when it transformed by Agrobacterium tumefaciens
determined by the bacteria Agrobacterium tumefaciens
of normal plant cells
both (a) and (b)

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Gene Transfer in Plants
Microprojectile method of gene transfer in plants involves delivery of DNA

with the help of micromanipulator
any of the above
with the help of needles
with the help of bolistics

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Gene Transfer in Plants
The size of the virulent plasmid of Agrobacterium tumefaciens is

40-80 kb
80-120 kb
>235 kb
140-235 kb

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