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Gene Transfer in Plants

Gene Transfer in Plants
The transfer of intermediate vectors into Agrobacterium are brought about by

transduction
biparental mating
transformation
triparental mating

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Gene Transfer in Plants
Which of the following is true about Agrobacterium tumefaciens?

All of these
It infects dicotyledonous angiosperms
It infects gymosperms
It causes crown gall disease of plants

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Gene Transfer in Plants
Which of the following is not true about the direct repeats flanking T-DNA?

All of these
These are important in integration mechanism
They are conserved between nopaline and octopine Ti-plasmids
These repeats are transferred intact to the plant genome

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Gene Transfer in Plants
In the liposome mediated gene transfer in plants, nucleic acids are

protected from nuclease digestion
stable in liposomes
not stable in liposomes
both (a) and (b)

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Gene Transfer in Plants
Direct DNA uptake by protoplasts can be stimulated by

all of these
polyethylene glycol (PEG)
decanal
luciferin

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Gene Transfer in Plants
Which of the following is not true about the helper plasmids?

These can't replicate in Agrobacterium
All of these
These can replicate in Agrobacterium
These help in the mediating conjugation of intermediate vectors

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