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Gene Transfer in Plants

Gene Transfer in Plants
Plant transformation vectors based on Agrobacterium can generally be divided into

eight vectors
four vectors
six vectors
two vectors

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Gene Transfer in Plants
In response to the activating signal molecule, an endonuclease is produced that causes nicks in the T-DNA. It is encoded by

vir C
vir D
vir B
vir A

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Gene Transfer in Plants
Intermediate vectors containing T-DNA are conjugation deficient. Thus conjugation is mediated in presence of which of the following plasmid?

either (a) or (b)
none of these
pRK 2013
pRN 3

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Gene Transfer in Plants
Which of the following is not true about the direct repeats flanking T-DNA?

These are important in integration mechanism
These repeats are transferred intact to the plant genome
All of these
They are conserved between nopaline and octopine Ti-plasmids

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Gene Transfer in Plants
Which of the following plant signal molecules regulate the expression of vir B, C, D and E in case of tobacco?

None of these
Both (a) and (b)
Acetosyringone
α-hydroxy syringone

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Gene Transfer in Plants
Virulence trait of Agrobacterium tumefaciens is borne on

chromosomal DNA
both chromosomal and plasmid DNA
cryptic plasmid DNA
tumour inducing plasmid DNA

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