Gene Transfer in Plants
Opine synthesis is the property

both (a) and (b)
conferred to plant cells when it transformed by Agrobacterium tumefaciens
determined by the bacteria Agrobacterium tumefaciens
of normal plant cells

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Gene Transfer in Plants
In a plant tumour cell

may vary from plant to plant
different parts of the Ti-plasmid are incorporated
complete Ti plasmid is incorporated in plant nuclear DNA
only a small specific segment of callus T DNA is incorporated

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Gene Transfer in Plants
Advantage of microprojectile method over microinjection method for gene transfer in plants include

method is universal in its application irrespective of all shape, size, type and presence or absence of cell wall
gene can be transferred to many cells simultaneously
All of these
intact cells are used

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