Gene Transfer in Plants
Because of large size of Ti-plasmid, intermediate vectors (IV) are developed in which T DNA has been subcloned into

all of these
pRK 2013
pRN 3
pBR 322 based plasmid vector

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Gene Transfer in Plants
Which of the following is true about T DNA?

Integration of T DNA can occur at many different, apparently random, sites in the plant nuclear DNA
Integration of T DNA occurs at one site that may be random in the plant nuclear DNA
Integration of T DNA occurs only at one specific sites in the plant nuclear DNA
Integration of T DNA occurs at two specific sites in the plant nuclear DNA

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Gene Transfer in Plants
Advantage of microprojectile method over microinjection method for gene transfer in plants include

intact cells are used
method is universal in its application irrespective of all shape, size, type and presence or absence of cell wall
gene can be transferred to many cells simultaneously
All of these

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