Gene Transfer in Plants
Because of large size of Ti-plasmid, intermediate vectors (IV) are developed in which T DNA has been subcloned into

pRN 3
all of these
pBR 322 based plasmid vector
pRK 2013

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Gene Transfer in Plants
Advantage of microprojectile method over microinjection method for gene transfer in plants include

gene can be transferred to many cells simultaneously
intact cells are used
All of these
method is universal in its application irrespective of all shape, size, type and presence or absence of cell wall

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Gene Transfer in Plants
In a plant tumour cell

may vary from plant to plant
only a small specific segment of callus T DNA is incorporated
complete Ti plasmid is incorporated in plant nuclear DNA
different parts of the Ti-plasmid are incorporated

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Gene Transfer in Plants
Crown gall tissue

retains tumorous properties when cultivated
shows tumorous properties only in presence of bacteria
can be cultivated in vitro in absence of bacteria
both (a) and (b)

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