Gene Transfer in Plants
Because of large size of Ti-plasmid, intermediate vectors (IV) are developed in which T DNA has been subcloned into

pRN 3
pRK 2013
pBR 322 based plasmid vector
all of these

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Gene Transfer in Plants
Crown gall tissue

shows tumorous properties only in presence of bacteria
can be cultivated in vitro in absence of bacteria
both (a) and (b)
retains tumorous properties when cultivated

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