Gene Transfer in Plants
Because of large size of Ti-plasmid, intermediate vectors (IV) are developed in which T DNA has been subcloned into

pBR 322 based plasmid vector
all of these
pRK 2013
pRN 3

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Gene Transfer in Plants
Virulent strains of Agrobacterium contain large Ti-plasmids, which are responsible for the DNA transfer and subsequent disease symptoms. It has been shown that Ti-plasmids contain

two sets of sequence necessary for gene transfer
four sets of sequence necessary for gene transfer
one set of sequence necessary for gene transfer
three sets of sequence necessary for gene transfer

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