Gene Transfer in Plants
Because of large size of Ti-plasmid, intermediate vectors (IV) are developed in which T DNA has been subcloned into

pRN 3
pBR 322 based plasmid vector
all of these
pRK 2013

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Gene Transfer in Plants
Opines are

amino acid derivatives found in normal tissues
amino acid derivatives found in both normal as well as tumor tissues
None of these
amino acid derivatives found in tumor tissues

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