Gene Transfer in Plants
Because of large size of Ti-plasmid, intermediate vectors (IV) are developed in which T DNA has been subcloned into

all of these
pRN 3
pBR 322 based plasmid vector
pRK 2013

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Gene Transfer in Plants
Advantage of microprojectile method over microinjection method for gene transfer in plants include

gene can be transferred to many cells simultaneously
All of these
intact cells are used
method is universal in its application irrespective of all shape, size, type and presence or absence of cell wall

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Gene Transfer in Plants
In a plant tumour cell

different parts of the Ti-plasmid are incorporated
only a small specific segment of callus T DNA is incorporated
may vary from plant to plant
complete Ti plasmid is incorporated in plant nuclear DNA

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