Gene Transfer in Plants
In a plant tumour cell

different parts of the Ti-plasmid are incorporated
may vary from plant to plant
complete Ti plasmid is incorporated in plant nuclear DNA
only a small specific segment of callus T DNA is incorporated

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Gene Transfer in Plants
Which of the following is not true for microinjection technique that involves transfer of DNA into protoplast?

It is carried out with the help of micromanipulator
It employs needle with diameter greater than cell diameter
All of these
The recipient cells are immobilized on artificial support or artificially bound to substarate

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Gene Transfer in Plants
Crown gall tissue

retains tumorous properties when cultivated
both (a) and (b)
can be cultivated in vitro in absence of bacteria
shows tumorous properties only in presence of bacteria

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Gene Transfer in Plants
Virulent strains of Agrobacterium contain large Ti-plasmids, which are responsible for the DNA transfer and subsequent disease symptoms. It has been shown that Ti-plasmids contain

three sets of sequence necessary for gene transfer
two sets of sequence necessary for gene transfer
one set of sequence necessary for gene transfer
four sets of sequence necessary for gene transfer

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