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Gene Transfer in Plants

Gene Transfer in Plants
Vir genes required for the T-DNA transfer and processing are located

none of these
on the T-DNA
outside the T-DNA region
on the plant genome

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Gene Transfer in Plants
Because of large size of Ti-plasmid, intermediate vectors (IV) are developed in which T DNA has been subcloned into

pBR 322 based plasmid vector
pRK 2013
all of these
pRN 3

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Gene Transfer in Plants
Direct DNA uptake by protoplasts can be stimulated by

decanal
polyethylene glycol (PEG)
all of these
luciferin

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Gene Transfer in Plants
Which of the following is true about Agrobacterium tumefaciens?

All of these
It causes crown gall disease of plants
It infects dicotyledonous angiosperms
It infects gymosperms

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Gene Transfer in Plants
Intermediate vectors containing T-DNA are conjugation deficient. Thus conjugation is mediated in presence of which of the following plasmid?

either (a) or (b)
none of these
pRK 2013
pRN 3

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Gene Transfer in Plants
The size of the virulent plasmid of Agrobacterium tumefaciens is

80-120 kb
140-235 kb
40-80 kb
>235 kb

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