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Gene Transfer in Plants

Gene Transfer in Plants
Vir genes required for the T-DNA transfer and processing are located

on the plant genome
none of these
outside the T-DNA region
on the T-DNA

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Gene Transfer in Plants
Co-integrating transformation vectors must include a region of homology in

the Ti-plasmid
the vector plasmid
none of these
between vector plasmid and Ti-plasmid

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Gene Transfer in Plants
Agrobacterium tumefaciens is a

a fungi
gram (-) bacteria
gram (+) bacteria
a yeast

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Gene Transfer in Plants
The size of the virulent plasmid of Agrobacterium tumefaciens is

40-80 kb
140-235 kb
80-120 kb
>235 kb

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Gene Transfer in Plants
Intermediate vectors containing T-DNA are conjugation deficient. Thus conjugation is mediated in presence of which of the following plasmid?

pRN 3
either (a) or (b)
none of these
pRK 2013

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Gene Transfer in Plants
Which of the following is not true about the helper plasmids?

These can replicate in Agrobacterium
All of these
These help in the mediating conjugation of intermediate vectors
These can't replicate in Agrobacterium

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