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Gene Transfer in Plants

Gene Transfer in Plants
Integrated nopaline T-DNA occurs as

four segments
single segment
three segments
two segments

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Gene Transfer in Plants
Which of the following is not true about the helper plasmids?

These can't replicate in Agrobacterium
These help in the mediating conjugation of intermediate vectors
All of these
These can replicate in Agrobacterium

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Gene Transfer in Plants
Because of large size of Ti-plasmid, intermediate vectors (IV) are developed in which T DNA has been subcloned into

all of these
pBR 322 based plasmid vector
pRK 2013
pRN 3

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Gene Transfer in Plants
Which technique is used to introduce genes into dicots?

Ti plasmid infection
microinjection
electroporation
particle acceleration

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Gene Transfer in Plants
The left segment of octopine T-DNA (TL) is necessary for

conjugative transfer
all of these
tumour formation
enzymes for agropine biosynthesis

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Gene Transfer in Plants
Which of the following is not true about the direct repeats flanking T-DNA?

These are important in integration mechanism
All of these
They are conserved between nopaline and octopine Ti-plasmids
These repeats are transferred intact to the plant genome

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