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Gene Transfer in Plants

Gene Transfer in Plants
Integrated nopaline T-DNA occurs as

single segment
three segments
four segments
two segments

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Gene Transfer in Plants
Vir genes required for the T-DNA transfer and processing are located

outside the T-DNA region
on the plant genome
on the T-DNA
none of these

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Gene Transfer in Plants
In response to the activating signal molecule, an endonuclease is produced that causes nicks in the T-DNA. It is encoded by

vir D
vir B
vir C
vir A

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Gene Transfer in Plants
Which of the following plant signal molecules regulate the expression of vir B, C, D and E in case of tobacco?

α-hydroxy syringone
Both (a) and (b)
None of these
Acetosyringone

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Gene Transfer in Plants
On Ti-plasmid T-region or T-DNA is flanked by a direct repeat of

30 bp
12 bp
25 bp
20 bp

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Gene Transfer in Plants
Intermediate vectors containing T-DNA are conjugation deficient. Thus conjugation is mediated in presence of which of the following plasmid?

pRK 2013
either (a) or (b)
pRN 3
none of these

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