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Gene Transfer in Plants

Gene Transfer in Plants
In response to the activating signal molecule, an endonuclease is produced that causes nicks in the T-DNA. It is encoded by

vir A
vir B
vir D
vir C

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Gene Transfer in Plants
Intermediate vectors containing T-DNA are conjugation deficient. Thus conjugation is mediated in presence of which of the following plasmid?

pRN 3
either (a) or (b)
none of these
pRK 2013

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Gene Transfer in Plants
The transfer of intermediate vectors into Agrobacterium are brought about by

triparental mating
transduction
biparental mating
transformation

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Gene Transfer in Plants
Which of the following is not true about the helper plasmids?

These can replicate in Agrobacterium
These can't replicate in Agrobacterium
These help in the mediating conjugation of intermediate vectors
All of these

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Gene Transfer in Plants
Which technique is used to introduce genes into dicots?

microinjection
Ti plasmid infection
electroporation
particle acceleration

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Gene Transfer in Plants
Which of the following is true about Agrobacterium tumefaciens?

It infects dicotyledonous angiosperms
All of these
It causes crown gall disease of plants
It infects gymosperms

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