Gene Transfer in Plants
Microinjection involves

injection of large amount of DNA
All of these
injection of DNA into bigger cells
injection with needle having diameter greater than cell diameter

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Gene Transfer in Plants
Virulent strains of Agrobacterium contain large Ti-plasmids, which are responsible for the DNA transfer and subsequent disease symptoms. It has been shown that Ti-plasmids contain

two sets of sequence necessary for gene transfer
three sets of sequence necessary for gene transfer
four sets of sequence necessary for gene transfer
one set of sequence necessary for gene transfer

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Gene Transfer in Plants
Crown gall tissue

both (a) and (b)
can be cultivated in vitro in absence of bacteria
retains tumorous properties when cultivated
shows tumorous properties only in presence of bacteria

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