Gene Transfer in Plants
Which of the following is not true about the direct repeats flanking T-DNA?

They are conserved between nopaline and octopine Ti-plasmids
All of these
These are important in integration mechanism
These repeats are transferred intact to the plant genome

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Gene Transfer in Plants
Which of the following is true about T DNA?

Integration of T DNA occurs at two specific sites in the plant nuclear DNA
Integration of T DNA occurs only at one specific sites in the plant nuclear DNA
Integration of T DNA occurs at one site that may be random in the plant nuclear DNA
Integration of T DNA can occur at many different, apparently random, sites in the plant nuclear DNA

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Gene Transfer in Plants
Virulent strains of Agrobacterium contain large Ti-plasmids, which are responsible for the DNA transfer and subsequent disease symptoms. It has been shown that Ti-plasmids contain

three sets of sequence necessary for gene transfer
one set of sequence necessary for gene transfer
two sets of sequence necessary for gene transfer
four sets of sequence necessary for gene transfer

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Gene Transfer in Plants
Crown gall tissue

both (a) and (b)
shows tumorous properties only in presence of bacteria
retains tumorous properties when cultivated
can be cultivated in vitro in absence of bacteria

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