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Gene Transfer in Plants

Gene Transfer in Plants
Agrobacterium tumefaciens is a

gram (-) bacteria
a yeast
a fungi
gram (+) bacteria

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Gene Transfer in Plants
Co-integrating transformation vectors must include a region of homology in

the Ti-plasmid
none of these
between vector plasmid and Ti-plasmid
the vector plasmid

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Gene Transfer in Plants
Liposomes mediated gene transfer in plants involves

fusion of liposomes with protoplast
All of these
plasmid DNA enclosed in a lipid bag
use of polyethylene glycol (PEG)

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Gene Transfer in Plants
On Ti-plasmid T-region or T-DNA is flanked by a direct repeat of

12 bp
30 bp
25 bp
20 bp

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Gene Transfer in Plants
Opine synthesis is the property

both (a) and (b)
determined by the bacteria Agrobacterium tumefaciens
of normal plant cells
conferred to plant cells when it transformed by Agrobacterium tumefaciens

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Gene Transfer in Plants
Because of large size of Ti-plasmid, intermediate vectors (IV) are developed in which T DNA has been subcloned into

pBR 322 based plasmid vector
pRK 2013
all of these
pRN 3

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