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Gene Transfer in Plants

Gene Transfer in Plants
Agrobacterium tumefaciens is a

a yeast
gram (+) bacteria
gram (-) bacteria
a fungi

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Gene Transfer in Plants
Microinjection involves

injection of DNA into bigger cells
injection with needle having diameter greater than cell diameter
injection of large amount of DNA
All of these

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Gene Transfer in Plants
Plant transformation vectors based on Agrobacterium can generally be divided into

four vectors
two vectors
eight vectors
six vectors

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Gene Transfer in Plants
Intermediate vectors containing T-DNA are conjugation deficient. Thus conjugation is mediated in presence of which of the following plasmid?

pRN 3
none of these
pRK 2013
either (a) or (b)

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Gene Transfer in Plants
Which of the following is not true about the helper plasmids?

All of these
These can replicate in Agrobacterium
These can't replicate in Agrobacterium
These help in the mediating conjugation of intermediate vectors

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Gene Transfer in Plants
On Ti-plasmid T-region or T-DNA is flanked by a direct repeat of

12 bp
25 bp
20 bp
30 bp

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