Gene Transfer in Plants
Virulent strains of Agrobacterium contain large Ti-plasmids, which are responsible for the DNA transfer and subsequent disease symptoms. It has been shown that Ti-plasmids contain

two sets of sequence necessary for gene transfer
three sets of sequence necessary for gene transfer
one set of sequence necessary for gene transfer
four sets of sequence necessary for gene transfer

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Gene Transfer in Plants
Crown gall tissue

can be cultivated in vitro in absence of bacteria
shows tumorous properties only in presence of bacteria
both (a) and (b)
retains tumorous properties when cultivated

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Gene Transfer in Plants
In a plant tumour cell

different parts of the Ti-plasmid are incorporated
may vary from plant to plant
only a small specific segment of callus T DNA is incorporated
complete Ti plasmid is incorporated in plant nuclear DNA

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