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Gene Transfer in Plants

Gene Transfer in Plants
Agrobacterium tumefaciens is a

gram (-) bacteria
a fungi
gram (+) bacteria
a yeast

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Gene Transfer in Plants
Which of the following is not true about the helper plasmids?

These help in the mediating conjugation of intermediate vectors
These can't replicate in Agrobacterium
These can replicate in Agrobacterium
All of these

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Gene Transfer in Plants
The left segment of octopine T-DNA (TL) is necessary for

conjugative transfer
all of these
enzymes for agropine biosynthesis
tumour formation

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Gene Transfer in Plants
Integrated octopine T DNA occurs as

single segment
two segments
four segments
three segments

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Gene Transfer in Plants
Intermediate vectors containing T-DNA are conjugation deficient. Thus conjugation is mediated in presence of which of the following plasmid?

none of these
either (a) or (b)
pRN 3
pRK 2013

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Gene Transfer in Plants
T-DNA transfer and processing into plant genome requires products of which of the following genes?

vir D,E
vir G,C
vir A,B
All of these

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