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Gene Transfer in Plants

Gene Transfer in Plants
Agrobacterium tumefaciens is a

gram (+) bacteria
a yeast
a fungi
gram (-) bacteria

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Gene Transfer in Plants
The transfer of intermediate vectors into Agrobacterium are brought about by

triparental mating
biparental mating
transduction
transformation

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Gene Transfer in Plants
The right segment of octopine T-DNA (TR) is necessary for

conjugative transfer
enzymes for agropine biosynthesis
all of these
tumour formation

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Gene Transfer in Plants
Which of the following is not true about the helper plasmids?

These can't replicate in Agrobacterium
These can replicate in Agrobacterium
These help in the mediating conjugation of intermediate vectors
All of these

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Gene Transfer in Plants
Direct DNA uptake by protoplasts can be stimulated by

polyethylene glycol (PEG)
luciferin
decanal
all of these

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Gene Transfer in Plants
Because of large size of Ti-plasmid, intermediate vectors (IV) are developed in which T DNA has been subcloned into

pBR 322 based plasmid vector
pRK 2013
all of these
pRN 3

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