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Gene Transfer in Plants

Gene Transfer in Plants
Agrobacterium tumefaciens is a

gram (-) bacteria
gram (+) bacteria
a fungi
a yeast

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Gene Transfer in Plants
Which of the following is not true about the direct repeats flanking T-DNA?

All of these
These are important in integration mechanism
They are conserved between nopaline and octopine Ti-plasmids
These repeats are transferred intact to the plant genome

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Gene Transfer in Plants
In response to the activating signal molecule, an endonuclease is produced that causes nicks in the T-DNA. It is encoded by

vir C
vir D
vir A
vir B

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Gene Transfer in Plants
Opines that are present in crown gall tumour include

all of these
agropine
nopaline
octopine

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Gene Transfer in Plants
Which of the following are used as selection marker for the cells transformed with Agrobacterium?

Streptomycin phosphotransferase
Neomycin phosphotransferase
Hygromycin phosphotransferase
any of the above

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Gene Transfer in Plants
The size of the virulent plasmid of Agrobacterium tumefaciens is

>235 kb
140-235 kb
40-80 kb
80-120 kb

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