Enzymes
The cleavage specificity of trypsin and chymotrypsin depend in part on the

size, shape, and charge of the active site or specificity pocket
presence of a low-barrier hydrogen bond in the active site or specificity pocket
absence of water in the active site
proximity of Ser 195 to the active site or specificity pocket

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Enzymes
The E.coli pyruvic acid dehydrogenase complex is reported to

Catalyze the reduction of pyruvic acid to acetyl Co A and CO2
retard the reduction of pyruvic acid to acetyl Co A and CO2
Catalyze the oxidation of pyruvic acid to acetyl Co A and CO2
decatalyze the oxidation of pyruvic acid to acetyl Co A and CO2

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Enzymes
Before they can react, many molecules need to be destabilized. This state is typically achieved through

the input of a small amount of activation energy
oxidizing the molecules by removing electrons
changing the three-dimensional shape of the molecule
changing the reaction from a biosynthetic to a catabolic pathway

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