Enzymes
Before they can react, many molecules need to be destabilized. This state is typically achieved through

changing the three-dimensional shape of the molecule
changing the reaction from a biosynthetic to a catabolic pathway
oxidizing the molecules by removing electrons
the input of a small amount of activation energy

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Enzymes
The cleavage specificity of trypsin and chymotrypsin depend in part on the

proximity of Ser 195 to the active site or specificity pocket
size, shape, and charge of the active site or specificity pocket
absence of water in the active site
presence of a low-barrier hydrogen bond in the active site or specificity pocket

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Enzymes
Enzyme-driven metabolic pathways can be made more efficient by

All of these
grouping enzymes into free-floating, multienzyme complexes
fixing enzymes into membranes so that they are adjacent to each other
concentrating enzymes within specific cellular compartments

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