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CLONING VECTORS

Cloning Vectors
Cos site of the cosmids

helps whole genome in circularization and ligation
consists of 12 bases
contains cleavage site
both (a) and (b)

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Cloning Vectors
M 13 is an example of

both (a) and (b)
filamentous phage
plasmid
single stranded DNA vector

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Cloning Vectors
Phagemid vectors are

combination of phages and cosmid
All of these
phages carrying properties of plasmids
combination of plasmid and phage λ

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Cloning Vectors
Single stranded vectors are useful

for oligonucleotide directed mutagenesis
All of these
for sequencing of cloned DNA
for probe preparation

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Cloning Vectors
Inserted DNA in λ gt 11 can be expressed as

free protein in the cytoplasm
All of these
β-galactosidase fused protein
free protein that is secreted out

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