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CLONING VECTORS

Cloning Vectors
Cos site of the cosmids

contains cleavage site
helps whole genome in circularization and ligation
consists of 12 bases
both (a) and (b)

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Cloning Vectors
M 13 is an example of

single stranded DNA vector
plasmid
both (a) and (b)
filamentous phage

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Cloning Vectors
Phagemid vectors are

combination of plasmid and phage λ
phages carrying properties of plasmids
combination of phages and cosmid
All of these

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Cloning Vectors
Single stranded vectors are useful

All of these
for probe preparation
for oligonucleotide directed mutagenesis
for sequencing of cloned DNA

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Cloning Vectors
Inserted DNA in λ gt 11 can be expressed as

free protein that is secreted out
All of these
free protein in the cytoplasm
β-galactosidase fused protein

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