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CLONING VECTORS

Cloning Vectors
Cos site of the cosmids

helps whole genome in circularization and ligation
both (a) and (b)
contains cleavage site
consists of 12 bases

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Cloning Vectors
M 13 is an example of

single stranded DNA vector
both (a) and (b)
filamentous phage
plasmid

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Cloning Vectors
Phagemid vectors are

All of these
combination of phages and cosmid
combination of plasmid and phage λ
phages carrying properties of plasmids

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Cloning Vectors
Single stranded vectors are useful

for oligonucleotide directed mutagenesis
for probe preparation
All of these
for sequencing of cloned DNA

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Cloning Vectors
Inserted DNA in λ gt 11 can be expressed as

All of these
free protein that is secreted out
free protein in the cytoplasm
β-galactosidase fused protein

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