Media Required for Plant Cell Culture
Nitrogen in the plant cell culture media is provided by either ammonia or nitrate salt. In the media

utilization of nitrate cause culture pH to drop while utilization of ammonium cause culture pH to rise
utilization of both ammonium and nitrate result in rise in pH
utilization of ammonium cause culture pH to drop while utilization of nitrate cause culture pH to rise
utilization of both ammonium and nitrate result in drop in pH

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Media Required for Plant Cell Culture
What is 'nurse' or conditioned medium?

It is the medium added to nurse the callus culture
It is the liquid medium removed from the suspension of fast growing cells
It is the media full of growth factors used for the growth of cells
Both (a) and (b)

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