Media Required for Plant Cell Culture Which of the following is not true about nurse or conditioned medium? It contains uncharacterized growth factor released by growing cells It is liquid removed from the suspension of fast growing cells it is removed aseptically from the culture and is autoclaved before use It is used in the culture of regenerating protoplast It contains uncharacterized growth factor released by growing cells It is liquid removed from the suspension of fast growing cells it is removed aseptically from the culture and is autoclaved before use It is used in the culture of regenerating protoplast ANSWER DOWNLOAD EXAMIANS APP
Media Required for Plant Cell Culture What is 'nurse' or conditioned medium? Both (a) and (b) It is the media full of growth factors used for the growth of cells It is the liquid medium removed from the suspension of fast growing cells It is the medium added to nurse the callus culture Both (a) and (b) It is the media full of growth factors used for the growth of cells It is the liquid medium removed from the suspension of fast growing cells It is the medium added to nurse the callus culture ANSWER DOWNLOAD EXAMIANS APP
Media Required for Plant Cell Culture To maintain the pH of the culture organic acid such as citric, fumaric, malic and succinic acid is used synthetic buffers such as Tris, MES or HEPS are used both (a) and (b) ammonium salts are used organic acid such as citric, fumaric, malic and succinic acid is used synthetic buffers such as Tris, MES or HEPS are used both (a) and (b) ammonium salts are used ANSWER DOWNLOAD EXAMIANS APP
Media Required for Plant Cell Culture Which of the following is not a cytokinin? Zeatin 6 benzylaminopurine Kinetin 2,4-dichlorophenoxyacetic acid Zeatin 6 benzylaminopurine Kinetin 2,4-dichlorophenoxyacetic acid ANSWER DOWNLOAD EXAMIANS APP
Media Required for Plant Cell Culture Silver thiosulphate is added to culture medium as it helps to prevent the gaseous plant hormone, ethylene dioxide from accumulating to detrimental condition. maintain the pH All of these remove toxic phenolics from plant cells prevent the gaseous plant hormone, ethylene dioxide from accumulating to detrimental condition. maintain the pH All of these remove toxic phenolics from plant cells ANSWER DOWNLOAD EXAMIANS APP
Media Required for Plant Cell Culture Neutralized activated charcoal is occasionally added to young regenerating cultures to maintain the pH of the medium both (a) and (b) help to remove plants growth regulators introduced at an earlier stage remove toxic phenolics produced by the stressed plant cell maintain the pH of the medium both (a) and (b) help to remove plants growth regulators introduced at an earlier stage remove toxic phenolics produced by the stressed plant cell ANSWER DOWNLOAD EXAMIANS APP