Media Required for Plant Cell Culture
Which of the following is not true about nurse or conditioned medium?

It is liquid removed from the suspension of fast growing cells
it is removed aseptically from the culture and is autoclaved before use
It contains uncharacterized growth factor released by growing cells
It is used in the culture of regenerating protoplast

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Media Required for Plant Cell Culture
Neutralized activated charcoal is occasionally added to young regenerating cultures to

both (a) and (b)
maintain the pH of the medium
help to remove plants growth regulators introduced at an earlier stage
remove toxic phenolics produced by the stressed plant cell

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