Media Required for Plant Cell Culture
Neutralized activated charcoal is occasionally added to young regenerating cultures to

both (a) and (b)
remove toxic phenolics produced by the stressed plant cell
help to remove plants growth regulators introduced at an earlier stage
maintain the pH of the medium

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Media Required for Plant Cell Culture
Nitrogen in the plant cell culture media is provided by either ammonia or nitrate salt. In the media

utilization of nitrate cause culture pH to drop while utilization of ammonium cause culture pH to rise
utilization of ammonium cause culture pH to drop while utilization of nitrate cause culture pH to rise
utilization of both ammonium and nitrate result in drop in pH
utilization of both ammonium and nitrate result in rise in pH

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