Media Required for Plant Cell Culture
To maintain the pH of the culture

synthetic buffers such as Tris, MES or HEPS are used
organic acid such as citric, fumaric, malic and succinic acid is used
both (a) and (b)
ammonium salts are used

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Media Required for Plant Cell Culture
Neutralized activated charcoal is occasionally added to young regenerating cultures to

both (a) and (b)
maintain the pH of the medium
remove toxic phenolics produced by the stressed plant cell
help to remove plants growth regulators introduced at an earlier stage

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