Media Required for Plant Cell Culture
To maintain the pH of the culture

both (a) and (b)
organic acid such as citric, fumaric, malic and succinic acid is used
synthetic buffers such as Tris, MES or HEPS are used
ammonium salts are used

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Media Required for Plant Cell Culture
What is 'nurse' or conditioned medium?

It is the medium added to nurse the callus culture
It is the media full of growth factors used for the growth of cells
It is the liquid medium removed from the suspension of fast growing cells
Both (a) and (b)

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