Media Required for Plant Cell Culture
To maintain the pH of the culture

ammonium salts are used
synthetic buffers such as Tris, MES or HEPS are used
organic acid such as citric, fumaric, malic and succinic acid is used
both (a) and (b)

ANSWER DOWNLOAD EXAMIANS APP

Media Required for Plant Cell Culture
Which of the following is not true about nurse or conditioned medium?

it is removed aseptically from the culture and is autoclaved before use
It is used in the culture of regenerating protoplast
It is liquid removed from the suspension of fast growing cells
It contains uncharacterized growth factor released by growing cells

ANSWER DOWNLOAD EXAMIANS APP

Media Required for Plant Cell Culture
What is 'nurse' or conditioned medium?

It is the liquid medium removed from the suspension of fast growing cells
It is the medium added to nurse the callus culture
Both (a) and (b)
It is the media full of growth factors used for the growth of cells

ANSWER DOWNLOAD EXAMIANS APP

Media Required for Plant Cell Culture
Nitrogen in the plant cell culture media is provided by either ammonia or nitrate salt. In the media

utilization of ammonium cause culture pH to drop while utilization of nitrate cause culture pH to rise
utilization of both ammonium and nitrate result in rise in pH
utilization of both ammonium and nitrate result in drop in pH
utilization of nitrate cause culture pH to drop while utilization of ammonium cause culture pH to rise

ANSWER DOWNLOAD EXAMIANS APP