Media Required for Plant Cell Culture
To maintain the pH of the culture

ammonium salts are used
both (a) and (b)
organic acid such as citric, fumaric, malic and succinic acid is used
synthetic buffers such as Tris, MES or HEPS are used

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Media Required for Plant Cell Culture
Which of the following is not true about nurse or conditioned medium?

It contains uncharacterized growth factor released by growing cells
It is liquid removed from the suspension of fast growing cells
It is used in the culture of regenerating protoplast
it is removed aseptically from the culture and is autoclaved before use

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