Media Required for Plant Cell Culture
To maintain the pH of the culture

synthetic buffers such as Tris, MES or HEPS are used
organic acid such as citric, fumaric, malic and succinic acid is used
both (a) and (b)
ammonium salts are used

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Media Required for Plant Cell Culture
Which of the following is not true about nurse or conditioned medium?

It is liquid removed from the suspension of fast growing cells
It contains uncharacterized growth factor released by growing cells
It is used in the culture of regenerating protoplast
it is removed aseptically from the culture and is autoclaved before use

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Media Required for Plant Cell Culture
Neutralized activated charcoal is occasionally added to young regenerating cultures to

both (a) and (b)
remove toxic phenolics produced by the stressed plant cell
maintain the pH of the medium
help to remove plants growth regulators introduced at an earlier stage

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