Media Required for Plant Cell Culture
To maintain the pH of the culture

ammonium salts are used
synthetic buffers such as Tris, MES or HEPS are used
both (a) and (b)
organic acid such as citric, fumaric, malic and succinic acid is used

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Media Required for Plant Cell Culture
Nitrogen in the plant cell culture media is provided by either ammonia or nitrate salt. In the media

utilization of both ammonium and nitrate result in drop in pH
utilization of both ammonium and nitrate result in rise in pH
utilization of ammonium cause culture pH to drop while utilization of nitrate cause culture pH to rise
utilization of nitrate cause culture pH to drop while utilization of ammonium cause culture pH to rise

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