Media Required for Plant Cell Culture
Nitrogen in the plant cell culture media is provided by either ammonia or nitrate salt. In the media

utilization of ammonium cause culture pH to drop while utilization of nitrate cause culture pH to rise
utilization of nitrate cause culture pH to drop while utilization of ammonium cause culture pH to rise
utilization of both ammonium and nitrate result in drop in pH
utilization of both ammonium and nitrate result in rise in pH

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Media Required for Plant Cell Culture
What is 'nurse' or conditioned medium?

It is the media full of growth factors used for the growth of cells
Both (a) and (b)
It is the liquid medium removed from the suspension of fast growing cells
It is the medium added to nurse the callus culture

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Media Required for Plant Cell Culture
Neutralized activated charcoal is occasionally added to young regenerating cultures to

remove toxic phenolics produced by the stressed plant cell
help to remove plants growth regulators introduced at an earlier stage
both (a) and (b)
maintain the pH of the medium

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