Media Required for Plant Cell Culture
What is 'nurse' or conditioned medium?

It is the liquid medium removed from the suspension of fast growing cells
It is the medium added to nurse the callus culture
It is the media full of growth factors used for the growth of cells
Both (a) and (b)

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Media Required for Plant Cell Culture
Neutralized activated charcoal is occasionally added to young regenerating cultures to

help to remove plants growth regulators introduced at an earlier stage
both (a) and (b)
maintain the pH of the medium
remove toxic phenolics produced by the stressed plant cell

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Media Required for Plant Cell Culture
Which of the following is not true about nurse or conditioned medium?

it is removed aseptically from the culture and is autoclaved before use
It is liquid removed from the suspension of fast growing cells
It contains uncharacterized growth factor released by growing cells
It is used in the culture of regenerating protoplast

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Media Required for Plant Cell Culture
Nitrogen in the plant cell culture media is provided by either ammonia or nitrate salt. In the media

utilization of both ammonium and nitrate result in drop in pH
utilization of nitrate cause culture pH to drop while utilization of ammonium cause culture pH to rise
utilization of both ammonium and nitrate result in rise in pH
utilization of ammonium cause culture pH to drop while utilization of nitrate cause culture pH to rise

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