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Media Required for Plant Cell Culture

Media Required for Plant Cell Culture
Which of the following growth regulator cause plant cells to grow?

Auxins
Brassinosteroids
Cytokinins
Gibberellins

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Media Required for Plant Cell Culture
Concentration of sucrose generally used in plant cell culture media is

60-70 g/l
40-50 g/l
10-15 g/l
20-30 g/l

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Media Required for Plant Cell Culture
What are the macronutrients used in plant cell culture medium?

N, P, Ca, Na, Cl
N, P, K, S, Na
N, P, K, S, Ca
N, P, K, Ca, Cl

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Media Required for Plant Cell Culture
Which is/are the naturally occurring plant auxins?

2,4-dichlorophenoxyacetic acid
All of these
Naphthalenacetic acid (NAA)
Indole acetic acid (IAA)

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Media Required for Plant Cell Culture
Which is/are the disadvantage/(s) of using IAA in plant cell culture media?

Gets easily oxidized
Conjugated to inactive form by plant cells
All of these
It is unstable in solution

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Media Required for Plant Cell Culture
To maintain the pH of the culture

organic acid such as citric, fumaric, malic and succinic acid is used
both (a) and (b)
synthetic buffers such as Tris, MES or HEPS are used
ammonium salts are used

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