Media Required for Plant Cell Culture
Nitrogen in the plant cell culture media is provided by either ammonia or nitrate salt. In the media

utilization of both ammonium and nitrate result in rise in pH
utilization of nitrate cause culture pH to drop while utilization of ammonium cause culture pH to rise
utilization of both ammonium and nitrate result in drop in pH
utilization of ammonium cause culture pH to drop while utilization of nitrate cause culture pH to rise

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Media Required for Plant Cell Culture
Which of the following is not true about nurse or conditioned medium?

It is liquid removed from the suspension of fast growing cells
It contains uncharacterized growth factor released by growing cells
it is removed aseptically from the culture and is autoclaved before use
It is used in the culture of regenerating protoplast

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