Media Required for Plant Cell Culture
Very high sugar concentration (40-100 g/l) have been used

none of these
Both (a) and (b)
in specialized secondary metabolite production
to adjust the osmotic potential of the media in short term treatment for regeneration

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Media Required for Plant Cell Culture
Neutralized activated charcoal is occasionally added to young regenerating cultures to

maintain the pH of the medium
remove toxic phenolics produced by the stressed plant cell
both (a) and (b)
help to remove plants growth regulators introduced at an earlier stage

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Media Required for Plant Cell Culture
What is 'nurse' or conditioned medium?

It is the liquid medium removed from the suspension of fast growing cells
Both (a) and (b)
It is the medium added to nurse the callus culture
It is the media full of growth factors used for the growth of cells

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Media Required for Plant Cell Culture
Nitrogen in the plant cell culture media is provided by either ammonia or nitrate salt. In the media

utilization of both ammonium and nitrate result in rise in pH
utilization of both ammonium and nitrate result in drop in pH
utilization of nitrate cause culture pH to drop while utilization of ammonium cause culture pH to rise
utilization of ammonium cause culture pH to drop while utilization of nitrate cause culture pH to rise

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