Recombinant DNA
The Southern blotting technique depends on

similarities between the sequences of probe DNA and experimental DNA
similarities between the sequences of probe RNA and experimental RNA
similarities between the sequences of probe protein and experimental protein
the molecular mass of proteins

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Recombinant DNA
The advantage of using DNA polymerases from thermophilic organisms in PCR is that

the DNA polymerases of these bacteria never make mistakes while replicating DNA
the DNA polymerases of these bacteria can withstand the high temperatures needed to denature the DNA strands
the DNA polymerases of these bacteria are much faster than those from other organisms
All of these

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Recombinant DNA
Bacterial cells protect their own DNA from degradation by restriction endonucleases by

not producing any restriction endonucleases
deleting all recognition sites from the genome
methylating the DNA at the sites that the enzyme recognizes
having anti restriction endonucleases

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